Learn More
Applied Biosystems™ Sequence Detection Primer
Description
These unlabeled primers can be used with TaqMan™ probes in any real-time PCR application. Choose between dry and in-solution formatted delivery. All primers are desalted.
Sequence detection primers are unlabeled primers that can be used with TaqMan probes or SYBR Green dye for your real-time PCR research applications.
Features include:
- Choice of delivered scales
- For use in any real-time PCR or PCR application
- All sequence detection primers are desalted
Real-time PCR sequence detection primers are delivered in either dry or liquid format.
Guaranteed yield
The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.
Approximate ship time
4–6 days in North America, 5-10 days in Europe
We also offer:
- Custom TaqMan probes with a variety of options for dyes and quenchers.
- TaqMan MGB probes and primers available as predesigned and pre-formulated TaqMan Assays.
- Commercial manufacturing products and services, including OEM and GMP oligo manufacturing.
Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.
Specifications
Specifications
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Product Type | Sequencing Primer |
| Content And Storage | 1 tube containing an unlabeled primer in solution or dried down. Store at -15°C to -25°C |
| Purification Method | Desalted |
| Shipping Condition | Room Temperature |
| Primer | Unlabeled |
| Quantity | 10,000 pmol |
| Product Line | Applied Biosystems |
| For Use With (Application) | Real Time PCR Applications |
| Form | Liquid or Dried Down |
Frequently Asked Questions (FAQs)
Most pre-designed TaqMan assays are supplied as a 20x assay concentrate containing 18 uM of each primer and 5 uM of probe which provides a 900 nM of each primer and 250 nM of the probe (for gene expression assays) and 200 nM of each probe (for SNP genotyping assays) at the final 1x concentration.
In order to make a 20x working stock from custom oligos, the oligos need to be diluted as follows: To make a 20x Assay stock for Gene Expression assays, make 100 uM stocks of both forward and reverse primers, and TaqMan probe. Add 18 ul of each primer and 5 ul of the TaqMan probe, which gives 41 ul volume. Add 59 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan Gene Expression Assay. To make a 20x Assay stock for a Genotyping Assay, make 100 uM stocks of both forward and reverse primers, and both TaqMan probes. Add 18 ul of each primer and 4 ul of each TaqMan probe, which gives 44 ul volume. Add 56 ul of 1X TE to bring the volume to 100 ul of 20X TaqMan SNP Genotyping Assays.
The main difference between Custom Standard DNA Oligos and Sequence Detection Primer (Cat. Nos. 4304970, 4304971, 4304972) is that Sequence Detection Primers come in the guaranteed specific amount and concentration that is ordered. Whereas Custom DNA Oligos can be selected by the Synthesis Scale. The scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) refers to the starting scale and not the guaranteed final amount.
You can find more information about the Synthesis Scale by going to the Custom Standard DNA Oligos page and clicking on the question mark next to “Synthesis Scale.”
In addition, the sequence detection primers are desalted, whereas the custom DNA oligos can be purified by HPLC, PAGE, and Cartridge.
We do not have pre-designed forward and reverse primers for GAPDH to be used in a SYBR Green dye-based real-time PCR assay. You can order either Sequence Detection Primers or Custom Standard DNA Oligos.
For Research Use Only. Not for use in diagnostic procedures.