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Applied Biosystems™ SeqStudio™ 8 Flex Genetic Analyzer

Catalog No. A53627
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Catalog No. A53627 Supplier Applied Biosystems™ Supplier No. A53627

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

The Applied Biosystems SeqStudio 8 Flex Genetic Analyzer offers design improvements and technological advances to the capillary electrophoresis (CE) workflow.

The Applied Biosystems SeqStudio 8 Flex Genetic Analyzer offers design improvements and technological advances to the capillary electrophoresis (CE) workflow. It delivers gold-standard Sanger sequencing and fragment analysis data quality with increased flexibility, easier operation, and cloud connectivity to enable researchers to manage their workloads and work/life balance effectively. Designed for ultimate ease-of-use, this mid-throughput system offers high quality, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

Features of the SeqStudio 8 Flex Genetic Analyzer include:
• Space savings with true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
• Improve workload management with continuous plate loading and sample reprioritization
• Realize lab efficiencies with remote monitoring
• New, improved capillary arrays for simple and hassle-free handling
• Reduce downtime with integrated remote troubleshooting tools

Easy-to-use data collection software
The data collection software pre-installed on the on-board touch screen computer takes user-friendliness to a new level. It offers an intuitive dashboard design, simplified plate set-up, and embedded self-help videos for common operations so that new users can get up and running quickly.

Scheduling flexibility
Four-plate capacity, continuous plate loading, and urgent sample reprioritization provide both the largest mid-throughput capacity and complete scheduling flexibility, translating into extra time in your day.

Remote functionality
Our cloud-based Connect platform provides enhanced flexibility by allowing users to set up plate files, monitor instrument and consumables performance and run progress, and even perform some critical instrument functions from anywhere.

Stay connected to your data
The SeqStudio 8 Flex system offers an automated workflow to obtain and share results from existing secondary analysis applications on Connect. These web-based software applications include Peak Scanner, Microsatellite Analysis, NGS Confirmation, Quality Check and Variant Analysis. They are compatible with Mac and PC.

Peace of mind with Smart service
Minimize downtime with premium service offerings for the SeqStudio 8 Flex Genetic Analyzer. Smart Help allows users to contact technical support directly from the touchscreen, without the need to download files or spend time on the phone. Experienced technicians will analyze the request and respond according to the user’s preference (phone or email) and resolve the issue as quickly as possible.

Smart Remote Support is an immersive collaboration tool that allows customers to show issues quickly and efficiently to technical support for resolution, helping reduce instrument downtime and minimize the need for on-site visits.

High application versatility
Simple workflows, reliable assays and reagents, and intuitive software are available for a broad range of applications, including multiplexed PCR analysis, next-generation confirmation, species identification, STR genotyping, cell-line authentication, dsDNA quality control, plasmid sequencing, and genome editing analysis.

Upgrade capabilities when you need them
The SeqStudio 8 Flex Genetic Analyzer can be seamlessly upgraded to a SeqStudio 24 Flex Genetic Analyzer by a field service engineer to provide additional throughput as your workload increases.

Results you can trust
Well-to-well and instrument-to-instrument data accuracy is achieved with the proven Applied Biosystems capillary electrophoresis technology.

Specifications

Description SeqStudio™ 8 Flex
Capillary Length 36 or 50 cm
For Use With (Equipment) Thermal Cyclers
External Computer Not Included
Filtered Filtered
Format 96-well plates or 8-tube strips
High-throughput Compatibility No
Operating Environment 59–86°F (15–30°C), 20–80% relative humidity (non-condensing). Room temperature should not fluctuate ±2°C during an instrument run.
Polymer POP-4™, POP-6™, POP-7™
Power Requirements 100–240 ± 10% VAC; 50/60 Hz
Quantity Each
Shipping Condition –22° to 140°F (–30° to 60°C), 5–95% relative humidity
Sterility Non-sterile
Temperature Range (Metric) 18–70°C (Oven temperature control)
Dimensions 27.6 x 26.6 x 34.1 in. (70 x 67.5 x 86.5 cm)
Weight 253.5 lb. (115 kg)
Voltage 100/240 V
Total Service Coverage Standard 1-year warranty included, extended warranties available
Max. Power Electrophoresis Voltage Up to 20 kV
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How can I request assistance from Technical Support or Instrument Service for SeqStudio Flex Series Genetic Analyzers?

You can request assistance from Technical Support or Instrument Service on the SeqStudio Flex Series Genetic Analyzers via the Smart Help function. In order to use this function, access to Thermo Fisher Connect must be enabled. You can also contact Technical Support at techsupport@thermofisher.com or Service Support at instrumentsupport@thermofisher.com.

With the SeqStudio Flex Genetic Analyzer, I tried to start a run, but the instrument displays a notification that there is not enough storage space. What should I do?

The SeqStudio Flex Genetic Analyzer instrument will not run unless the required amount of space is available to save the results on the instrument. To free up space on the hard drive, you will need to delete the run histories. We recommend that you first export the results before deleting them as the process is irreversible. At the home screen, tap Actions > Run history. Select the plates that you want to delete. Tap Delete > OK to confirm.

In the SeqStudio Flex Genetic Analyzer, I do not have access to the CBC or capillary array because the CBC is currently beneath the array with the tips inserted. How can I move it so I can have access to the array or CBC?

You can move the CBC by using the Manual commands. Tap on Dashboard > Instrument Status > Manual commands. Select Move buffer container > Send command. Once the CBC is no longer beneath the array with the tips inserted, close the Manual commands screen. You will now have access to the array and CBC.

What should I do if the SeqStudio Flex Genetic Analyzer touchscreen becomes unresponsive?

If the SeqStudio Flex Genetic Analyzer touchscreen becomes unresponsive, press the power on/off button on the front of the instrument to restart the instrument. If the touchscreen is still unresponsive after the reboot, contact Technical Support at techsupport@thermofisher.com.

What should I do if the SeqStudio Flex Genetic Analyzer instrument panel is blinking amber?

If the SeqStudio Flex Genetic Analyzer instrument panel is blinking amber, you should restart the instrument.

What versions of desktop secondary analysis software can I use to analyze data generated from the SeqStudio Flex Genetic Analyzer?

The desktop secondary analysis software versions that are compatible with the SeqStudio Flex Series Genetic Analyzers are:

- Sequencing Analysis Software v7.1 or later
- SeqScape Software v4.1 or later
- Variant Reporter Software v3.1 or later
- Minor Variant Finder Software v1.2 or later
- MicrobeBridge Software v1.2 or later
- GeneMapper Software v6.1 or later

How many plates can the SeqStudio Flex Series Genetic Analyzers hold and how can I see which plate is running?

The SeqStudio Flex plate holder can hold up to 4 plates, corresponding to positions A, B, C, and D on the home screen of the software. When a plate is running, the console will display the status “Running” as well as the Time remaining on the run in the plate position. If a run has completed, the status “Completed” would be displayed with a glowing border around the plate position.

Will the SeqStudio Flex Genetic Analyzer instrument prevent a run from starting if the consumables have expired or exceeded their on-instrument shelf life?

By default, the SeqStudio Flex Genetic Analyzersare configured to allow the use of consumables beyond the expiration date and on-instrument shelf life. However, there are a couple of exceptions. The conditioning reagent is a single-use consumable. In the case of polymer, insufficient volume in the polymer pouch will prevent the run from starting. An administrator can change the configuration to prevent running if a consumable has expired or exceeded its on-instrument life. Please note that we do not recommend using consumables that exceed supported limits.

With the SeqStudio Flex Series Genetic Analyzers, can I open the drawer while a run is going on?

You can open the drawer at any time when the drawer is unlocked. When the drawer is unlocked, the screen will display “Drawer unlocked.” During sample injection, the drawer is locked, and the message displayed will be “Drawer locked.”

What is different about the SeqStudio Flex Series Genetic Analyzer plate retainers compared with the 3500 plate retainers? When shouldn't I use the 3500 plate retainers on the SeqStudio Flex Series Genetic Analyzers?

The SeqStudio Flex Series Genetic Analyzer plate retainers have one opening on the side to allow barcode reading while the 3500 plate retainers have two openings on the side which can cover the plate barcode. If the automatic barcode workflow is enabled on the SeqStudio Flex Series Genetic Analyzer, using the 3500 plate retainers will cause a barcode error in the home screen.

96-Well Standard Retainer & Base Set SeqStudio Flex Series: Cat. No. A49316
96-Well Fast Retainer & Base Set SeqStudio Flex Series: Cat. No. A49495
8-Tube Standard Retainer & Base Set SeqStudio Flex Series: Cat. No. A49296
8-Tube Fast (0.1 mL) Retainer & Base Set SeqStudio Flex Series: Cat. No. A49298
384-Well Retainer & Base Set SeqStudio Flex Series: Cat. No. A49496

Can the arrays for the 3500/3500xL Genetic Analyzers be used on the SeqStudio Flex Series Genetic Analyzers?

No, the arrays for the 3500/3500xL Genetic Analyzers cannot be used on the SeqStudio Flex Series Genetic Analyzers. The SeqStudio Flex Series Genetic Analyzercapillary array SKUs are listed below:

- Capillary array 36‑cm SeqStudio 8 Flex: Cat. No. A49104
- Capillary array 50‑cm SeqStudio 8 Flex: Cat. No. A49106
- Capillary array 36‑cm SeqStudio 24 Flex: Cat. No. A49105
- Capillary array 50‑cm SeqStudio 24 Flex: Cat. No. A49107

What consumables are shared between the SeqStudio Flex Series Genetic Analyzers and the 3500/3500xL Genetic Analyzers?

Consumables that are shared between the SeqStudio Flex Series Genetic Analyzers and the 3500/3500xL Genetic Analyzers include the Performance-Optimized-Polymer (POP), Anode Buffer Container (ABC), Cathode Buffer Container (CBC), and Conditioning Reagent.

Do I need to purchase a computer in order to run the SeqStudio Flex Series Genetic Analyzers?

There is no requirement to purchase a computer in order to run the SeqStudio Flex Series Genetic Analyzers. The Data Collection software is installed on the instrument. However, using secondary analysis software to analyze data and/or to run the Security, Audit, and Esignature (SAE) modules will require an additional computer.

What are the startup and shutdown procedures for the SeqStudio Flex Series Genetic Analyzers?

To power on the instrument, press the power on/off button on the front of the instrument. Make sure that the blue status light is on. To shut down the instrument, press the power button to turn off the instrument. If the instrument is idle for greater than 2 weeks, perform a long-term shutdown by following the Shut Down Wizard.

What are the specifications for the SeqStudio Flex Series Genetic Analyzers?

The specifications for the SeqStudio Flex Series Genetic Analyzers are:

Dimensions (W x D x H): 70 x 67.5 x 86.5 cm (uncrated)
Weight: ~115 kg
Number of Capillaries: 8 (Cat. No. A53627) or 24 (Cat. No. A53630)
Number of Dyes: 6
Sample Format: 96-well standard plates, 384-well standard plates, 96-well fast plates, 8-well standard strip tubes, and 8-well fast strip tubes

What is the SeqStudio Flex Genetic Analyzer System and what are some of its features?

The SeqStudio Flex Genetic Analyzer System is a medium-throughput fluorescence-based capillary electrophoresis system that is offered in two models. The SeqStudio 8 Flex Genetic Analyzer (Cat. No. A53627) holds the 8-capillary array and the SeqStudio 24 Flex Genetic Analyzer (Cat. No. A53630) holds the 24-capillary array. The available array lengths are 36 cm and 50 cm. The SeqStudio Flex Genetic Analyzer instruments use the Applied Biosystems SeqStudio Flex Genetic Analyzer Data Collection Software (SeqStudio Flex DCS) which resides on the instrument.

Features include:

- Onboard touchscreen computer
- 4 plate loading capacity and prioritization
- Automated barcode workflow
- Cloud connectivity capabilities enabling remote monitoring and remote plate file creation
- Voice commands
- Smart Help on-instrument support request
- Remote session capabilities


For Research Use Only.