Sigma-Aldrich Schiff′s Reagent Intense

The Schiff's reagent Intense, for the detection of aldehyde and mucosubstances in microscopy, is a ready-to-use solution used for human-medical cell diagnosis and serves the histological investigation of sample material of human origin, for example histological sections of e. g. the intestine or liver.

• This ready-to-use Schiff's reagent Intense can be used to stain mucopolysaccharides in histological tissue specimens. The PAS (periodic acid schiff) reaction is one of the most frequently used chemical methods for the detection of aldehyde and mucosubstances in microscopy.
• In the PAS reaction, the histological specimen material is first treated with periodic acid, resulting in the oxidation of the 1,2-glycols into aldehyde groups.
• The addition of Schiff's reagent (fuchsin-sulfuric acid) in the second step causes the aldehydes to react to form a brilliant red color.
• In the end result, the PAS reaction yields a specific color reaction with unsubstituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glyco- and phospholipids.
• The PAS reaction can be further combined with the Alcian blue staining method to detect mucosubstances (glycosaminoglycans).
• The 500 mL bottle is sufficient for 3000 stainings.
• Form: liquid
• IVD: for in vitro diagnostic use
• pH: 1.74 in H₂O
• Storage temperature: 20°C to 25°C.