Thermo Scientific™ Remel™ Potassium Hydroxide 20%

Description

Determine the ability of gram-negative bacilli to produce acetylmethylcarbinol (acetoin) from glucose fermentation using Thermo Scientific™ Remel™ 20% Potassium Hydroxide preparation. This reagent is recommended for use with MICRO-ID®. In 1898, Voges and Proskauer reported production of acetylmethylcarbinol from glucose fermentation by certain bacteria when grown in an appropriate medium such as MR-VP Broth1. MICRO-ID® utilizes a modification of Clark and Lubs medium containing glucose, sodium pyruvate, arginine, and an alpha naphthol derivative2.

Determine the ability of gram-negative bacilli to produce acetylmethylcarbinol (acetoin) from glucose fermentation using 20% Potassium Hydroxide preparation.Ready for Use: convenience of a prepared reagent.Ease of Differentiation: VP positive organisms produce a pink color development while VP negative organisms produce no color.Rapid Detection: within 10 minutes.The Voges-Proskauer test is based on the detection of acetylmethylcarbinol from glucose metabolism3.Glucose is metabolized to pyruvic acid from which the neutral products acetoin and 2,3-butanediol are formed. One molecule of acetoin is formed by the decarboxylation of two molecules of pyruvic acid. In the presence of oxygen and 20% KOH (alkali), acetoin and 2,3-butanediol are oxidized to diacetyl, the reactant for the pink color produced in Voges-Proskauer test3. This colored complex forms with arginine and alpha naphthol derivative.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.