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Thermo Scientific™ Middlebrook 7H9 Broth w/Glycerol

Catalog No. R061346
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10 x 2 mL
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R061346 10 x 2 mL
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Catalog No. R061346 Supplier Thermo Scientific™ Supplier No. RE061346
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Description

Isolate and cultivate Mycobacterium species using Thermo Scientific™ Remel™ Middlebrook 7H9 Broth w/Glycerol.

Isolate and cultivate Mycobacterium species using Thermo Scientific™ Remel™ Middlebrook 7H9 Broth w/Glycerol. In 1958, Middlebrook and Cohn first described Middlebrook 7H9 Broth for the cultivation of mycobacteria1. The supplemental nutrients glycerol, oleic acid, albumin, and dextrose were found to enhance the growtth of most mycobacteria2,3. This medium is used as a basal medium to prepare inocula for biochemical identification tests and antibiotic susceptibility testing procedures4,5.

Use Middlebrook 7H9 Broth with Glycerol for isolation and cultivation of Mycobacterium species.

  • Recommended for identification and susceptibility testing of Mycobacteria, Nocardiae and other aerobic Actinomycetes by CLSI5.
  • Ready-to-use – Convenience of prepared media.

Middlebrook 7H9 Broth with Glycerol contains inorganic compounds that supply potassium, sulfur, magnesium, and phosphorus necessary for the growth of mycobacteria. Inorganic copper, iron, zinc, and calcium stimulate growth. Sodium citrate converts to citric acid in solution and holds certain inorganic cations. Ammonium sulfate is a source of nitrogen, dextrose and glycerol provide carbon and albumin binds to the free fatty acids which may be toxic to Mycobacterium species. Catalase destroys toxic peroxides and catalyzes the reaction of iron with molecular oxygen. Biotin is required for carboxylation and decarboxylation reactions and pyridoxine is a precursor of enzymatic activity.

Specifications

Description Middlebrook 7H9 Broth w/Glycerol
Format 15mm x 103mm Tube
Product Type Broth Tube
Quantity 10 x 2 mL