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Thermo Scientific™ Gram-Sure

Catalog No. R211820
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25 Disks/Vial
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R211820 25 Disks/Vial
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Catalog No. R211820 Supplier Thermo Scientific™ Supplier No. RE211820
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Description

Use Gram-Sure reagent-impregnated disk to rapidly differentiate aerobic, Gram-negative, and Gram-positive rods or coccobacilli.

Differentiate aerobic, Gram-negative and Gram-positive rods or coccobacilli with Thermo Scientific™ Remel™ Gram-Sure reagent-impregnated disks. This is a rapid disk test performed using fluorescent technology. The Gram stain is generally the first step in the identification of bacteria and decides subsequent identification procedures. The tendency of some Gram-positive rods to appear Gram-variable or Gram-negative, especially members of the genera Bacillus, Erysipelothrix, Lactobacillus, and Listeria, may often result in misclassification.

Differentiate aerobic, Gram-negative and Gram-positive rods or coccobacilli with Gram-Sure Disks.

  • Ready to use - Reagent-impregnated disk. No further prepartion is necessary.
  • Rapid test - Incubate at room temperature for 5-10 minutes.
  • Easy to interpret - Gram-negative organisms (blue fluorescence under longwave ultraviolet light) are easy to differentiate from Gram-positive organisms (no fluorescence under longwave ultraviolet light).

The cell walls of Gram-negative organisms contain aminopeptidase, which is detected by hydrolysis of L-alanine-7-amido-4-methylcoumarin from a non-fluorescent substrate to a fluorescent compound. In 1976, Cerney reported using L-alanine-4-nitroanilide to detect cell-wall aminopeptidase, which correlated with Gram negativity1. In 1990, Manafi and Kneifel evaluated various chromogenic and fluorogenic substrates for the ability to differentiate Gram-positive and Gram-negative bacteria2. They recommended using the fluorogenic substrate L-alanine-7-amido-4-methylcoumarin due to its higher test sensitivity and pronounced blue fluorescence indicating a positive reaction.

Gram-Sure disk contains the reactive ingredient L-alanine-7-amido-4-methylcoumarin which exhibits pronounced blue fluorescence and increased test sensitivity. Aerobic, Gram-negative rods and coccobacilli show a bright blue fluorescence, whereas Gram-positive rods and coccobacilli do not display any fluorescence.

This test is used to serve as an adjunct to the traditional Gram stain method and not a replacement.

Specifications

Content And Storage 2°C to 8°C
Description Gram-Sure
Format Vial
Quantity 25 Disks/Vial