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Thermo Scientific™ ALA Disk

Catalog No. R21115
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25 Disks/Vial
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R21115 25 Disks/Vial
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Catalog No. R21115 Supplier Thermo Scientific™ Supplier No. RE21115
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Description

Rapidly differentiate Haemophilus species on the basis of the detection of porphyrin synthesis using ALA Disk.

Rapidly differentiate Haemophilus species on the basis of the detection of porphyrin synthesis using Thermo Scientific™ Remel™ ALA Disk. In 1974, Kilian described a rapid method for identification of X-factor independent Haemophilus spp. based on the ability to synthesize heme precursors from ALA1. Lund and Blazevic determined the porphyrin test is more rapid and accurate for identification of X-factor-independent strains of Haemophilus (e.g. H. parainfluenzae and H. parahaemolyticus) than the satellite test2.

Members of the genus Haemophilus are small non-motile Gram-negative bacilli traditionally defined by a requirement for X and/or V factor3. X factor is derived from hemoglobin and V factor through the satellite relationship with Staphylococcus aureus.

Rapidly differentiate Haemophilus species on the basis of their detection of porphyrin synthesis using ALA Disk.

  • Ready to use: Reagent-impregnated disk
  • Rapid test: Results are obtained within a few hours.
  • Accurate test: For detection of porphyrins which are only excreted by X-factor-independent Haemophilus spp.
  • Easy to identify: A positive result showcases bright yellow fluorescence under long wave ultraviolet light.

After incubation the disk is exposed to long wave ultraviolet light in a dark room for any indication of fluorescence. Biberstein et al. studied the action of Haemophilus on δ-aminolevulinic acid (ALA) and reported a perfect correlation between X-factor-independence and the ability to convert ALA to porphyrins4.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

Description ALA Disk™
Detectable Analytes Porphyrin
Type ALA Disk
Quantity 25 Disks/Vial