Invitrogen™ Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet™ 615, eBioscience™

Rat Monoclonal Isotype Control

The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Tested This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 350 nm; Emission: 615 nm; Laser: Ultraviolet Laser.....