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Gibco™ Rat Glial Precursor Cells

Catalog No. N7746100
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1 x 106 cells
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N7746100 1 x 106 cells
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Catalog No. N7746100 Supplier Gibco™ Supplier No. N7746100
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Description

Rat Glial Precursor Cells

Ready-to use system: Upon proliferation in StemPro™ NSC-SFM, >80% of the cells retain their undifferentiated Phenotype, good post-thaw cell viability (≥ 50%), and supports differentiation into Oligodendrocytes and Astrocytes. Gibco™ Rat Glial Precursor Cells (GPC) offer researchers ready access to high quality cells and eliminate the time and effort to isolate these highly sensitive cells in house. Glial Precursor Cells are isolated from the cortex of newborn Sprague-Dawley rats. The cells are isolated under sterile conditions, expanded in complete StemPro™ NSC SFM, and cryopreserved at passage 2 (P2). Each vial contains 1 × 106 cells/ml viable cells (post-thaw) that can be expanded once to give >2 fold increase of cells. Upon proliferation in StemPro™ NSC SFM, >80% of cells retain their undifferentiated Phenotype and can be differentiated into Oligodendrocytes and Astrocytes. Glial Precursor Cells are the restricted progenitors, and the majority of their down stream progeny are Oligodendrocytes and Astrocytes. Oligodendrocytes produce myelin to protect neurons and secure proper signal transduction. Demyelination is involved with critical diseases, such as multiple sclerosis, Addison-Schilder Disease (ALD) etc.

Specifications

Content And Storage Store in liquid nitrogen.
Age Newborn (14 days or less)
Cell Type Glial Precursor Cells
Species Rat
Tissue Cortex
No. of Cells 1 x 106
Quantity 1 x 106 cells
Tested For Cell Purity, Differentiation Potential, Sterility, Viability, Mycoplasma

Frequently Asked Questions (FAQs)

How long can I culture Gibco Rat Glial Precursor Cells?

These cells can be thawed and passaged once before using in experiments. The single passage will yield a 2-fold increase of thawed cells. Cells do not expand significantly beyond first passage post-thaw.
Where were Gibco Rat Glial Precursor Cells (Cat. No. N7746100) isolated from? How many passages do these cells go through before being cryopreserved?

Rat Glial Precursor Cells were isolated from the cortex of new born Sprague Dawley rats at day 2 after birth, and cryopreserved at passage 2.

What are the growth factors or cytokines used in neural stem cell (NSC) culture?

For NSC expansion, the following growth factors are used: recombinant EGF (Cat. No. PHG0314), recombinant FGF-basic (Cat. No. PHG0024), and recombinant VEGF (Cat. No. PHC9394). In addition, several neurotrophins such as BDNF Cat. No. 10908010), NT-3, CNTF (Cat. No. PHC7015), and GDNF (Cat. No. PHC7044)are also used in the related studies.

Information pertaining to whether a specific product has been tested against the WHO Reference Standard can typically be located on the product page or Certificate of Analysis (COA).
How can I culture human neural stem cells (NSCs)?

Human NSCs can grow in Gibco StemPro NSC SFM (Cat. No. A1050901) on dishes pre-coated with Gibco Geltrex Matrix or Gibco CELLstart substrate. Alternatively, if the goal is to obtain neurons, NSCs can also be grown on Neurobasal medium supplemented with Gibco B-27 supplements without vitamin A on a pre-coated dish.
How can I characterize neural stem cells (NSCs)?

NSCs are generally characterized by their ability to form neurospheres when plated at cloning density (Nat Methods 2:333 (2005)). NSCs can also be characterized by (1) RT-PCR of Sox1, Sox2, and Nestin or (2) immunohistochemical staining for nestin, Pax6, Sox2, and Ki67.

What are neural stem cells?

Neural stem cells (NSCs) are self-renewing multipotent cells of the nervous system capable of differentiating into neurons, oligodendrocytes, and astrocytes. NSC can be generated by induced differentiation from embryonic stem (ES) cells, or isolated from various regions of the brain including the cortex, the subventricular zone (SVZ), and the ventricular zone, or generated from bone marrow-derived mesenchymal stem cells (MSCs) (J Cell Biochem 114:764 (2013)). NSCs are valuable tools for the study of neurogenesis and neurotransmitter and receptor function. NSCs were used in the investigation of different CNS disorders such as PD and Huntington's disease in various animal models (J Cell Biochem 114:764 (2013)).

For Research Use Only. Not for use in diagnostic procedures.