Ultra-High Fidelity Including dNTP's RadiantTM HiFi Ultra DNA Polymerase is a high-performance, high-fidelity DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM HiFi Ultra Polymerase possesses 5-3 DNA polymerase and 3-5 proofreading exonuclease activities with an error-rate of 4.55 x 10-7 . The polymerase is ideal for cloning applications, library construction, screening and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM HiFi Ultra Polymerase is engineered for robust, superior high-fidelity PCR in comparison with standard proof-reading enzymes such as pfu DNA Polymerase. Several point-mutations combined with a new-generation buffer formulation provide for significantly improved processivity for faster cycling, greater sensitivity and less inhibition with crude DNA samples. The supplied 5X HiFi Ultra Reaction Buffer contains an ideal concentration of MgCl2 and
