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MilliporeSigma™ Rac1/cdc42 Activation Magnetic Beads Pulldown Assay

Catalog No. 1710394
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30 Assays
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Catalog No. Quantity
1710394 30 Assays
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Catalog No. 1710394 Supplier MilliporeSigma™ Supplier No. 1710394
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Description

Includes

  • Rac1/cdc42 Assay Reagent
  • Anti-Rac1, clone 23A8
  • Anti-cdc42, mouse monoclonal IgG1
  • Mg2+ Lysis/Wash Buffer, 5X
  • 100X GTPγS, 10mM
  • 100X GDP, 100mM

Requires

Stimulated and non-stimulated cell lysates pipettes, micropipettes and tips electrophoresis and immunoblotting equipment microcentrifuge microcentrifuge tubes Rac activators glutathione agarose tissue culture supplies 4C tube rocker 30C incubator or water bath glycerol 500 mM EDTA

Effective method for detecting Rac and Cdc42 activity in cell lysates

  • Rac1/cdc42 Activation Magnetic Beads Pulldown Assay provides an effective method for detecting Rac and Cdc42 activity in cell lysates
  • This assay uses the downstream effector of Rac/Cdc42, p21-activated protein kinase (PAK1), to isolated the active GTP-bound form of Rac/Cdc42 from the sample
  • The p21 binding domain (PBD) of PAK1 is expressed as a GST-fusion protein and coupled to magnetic beads
  • After magnetic rack separation, an immunoblot is performed and the activated Rac/Cdc42 is detected with specific monoclonal antibodies, followed by HRP-conjugated secondary antibody and ECL reagent

Specifications

Content And Storage 1 year at -20°C from date of shipment
For Use With (Application) Affinity Binding Assay, G-Protein Assay, Activity Assay
Quantity 30 Assays