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Applied Biosystems™ QuantStudio™ 3D Digital PCR Tilt Base for ProFlex™ Thermal Cycler

Catalog No. A24898
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Catalog No. A24898 Supplier Applied Biosystems™ Supplier No. A24898
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For ProFlex™ Thermal Cycler sits beneath the ProFlex™ Thermal Cycler and ensures consistent performance during PCR amplification of the QuantStudio™ 3D Digital PCR chips.

Simply affix the two risers and place the tilt base beneath the feet of the thermal cycler, load your prepared 3D Digital PCR chips, and you are ready for PCR amplification.

Specifications

Content And Storage Includes: 1 tilt base, 2 tilt base risers, and 12 riser cable ties
For Use With (Equipment) ProFlex™ PCR System, QuantStudio™ 3D PCR System
Product Type 3D Digital PCR Tilt Base
For Use With (Application) Real Time PCR (qPCR)
Quantity 1 each
How do I replace the fuses in the QuantStudio 3D Digital PCR System?

Please follow the procedures described in "Chapter A: Maintenance" of the QuantStudio 3D Digital PCR System User Guide to replace the fuses. Make sure to only replace with those listed and certified fuses of the same type and rating as those currently in the QuantStudio 3D instrument.

How does sample preparation for digital PCR differ from real-time PCR?

The answer is that sample prep for digital PCR is no different from real-time PCR when using the QuantStudio 3D Digital PCR System. If you have samples in your freezer that you've run on real-time PCR, then you're ready to run them with digital PCR.

How do I determine how much sample to use when setting up the digital PCR reaction for the QuantStudio 3D Digital PCR System?

Please see the section on Sample Dilution in Chapter 3 of the QuantStudio 3D Digital PCR System User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0007720.pdf) for more details on how to determine the target copy number per genome.

Where are my chip image data saved on the QuantStudio 3D instrument?

You can configure the QuantStudio 3D instrument to upload the imaging data to a USB drive, a network file server, or the cloud data storage service. See Appendix B, “Networking”, in the QuantStudio 3D Digital PCR System User Guide for more information on setting up network destinations. Also, the QuantStudio 3D instrument retains a copy of the analyzed data from the imaged QuantStudio 3D Digital PCR 20K Chip that you can access from the Run History screen. Note: The results will remain in the QuantStudio 3D instrument cache for approximately 300 readings. After 300 chips, the instrument removes the oldest data file in the cache to store each new reading.

For Research Use Only. Not for use in diagnostic procedures.