Promega™ Transcend™ Nonradioactive Translation Detection Systems
Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.
Manufacturer: Promega™ L5070
Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection.
- Sensitive: The biotin tag allows detection of 0.5 to 5ng of translated protein
- Safe: No radioisotope handling, storage or disposal is required
- Fast: Labeled proteins can be detected three to four hours after gel electrophoresis
- Flexible: Results can be visualized by using colorimetric or chemiluminescent detection
- IRNA available separately (PR-L5061)
|Non-radioactive detection of Proteins synthesized in-vitro|
|Transcend Colorimetric Nonradioactive Translation Detection System|
|COMPONENTS AT DIFFERENT TEMPS|
|30μL Transcend™ Trna, 36μL Streptavidin-AP, 35mL Western Blue™ stabilized substrate for Alkaline Phosphatase|
|30 x 50μL Reactions|