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Promega TNT™ Quick Coupled Transcription/Translation System

Catalog No. PRL1170
Encompass
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Product Type:
TNT SP6
TNT T7
No. of Reactions:
40
5
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Catalog No. Product Type No. of Reactions
PRL1170 TNT T7 40
PRL1171 TNT T7 5
PRL2080 TNT SP6 40
PRL2081 TNT SP6 5
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Catalog No. PRL1170 Supplier Promega Supplier No. L1170
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Description

The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.

  • Single-Tube, Coupled Transcription/Translation for Eukaryotic Cell-Free Protein Expression
  • Functional protein is expressed in one hour, ready for use without further purification
  • Use protein for protein:protein and protein:nucleic acid interactions, protein modification and more
  • Choice of SP6 or T7 promoter

  • U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289 and other patents.
  • U.S. Patents 5,324,637 and 5,492,817; Australian Patent 660329 and other patents.
  • U.S. Patent 5,552,302; Australian Patent 646803 and other patents.
  • The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
  • Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.
  • For Laboratory Use. Any use of the product for diagnostics requiring clearance or approval by the FDA may require a license under Mayo Clinic U.S. Patents 6,027,913 and 6,361,949.

Specifications

Content And Storage <-65°C
For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
Includes 8 × 200μL TNT Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 250μL Luciferase Assay Reagent, 50μL Methionine, 1250μL Nuclease-free Water
No. of Reactions 40
Product Type TNT T7