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Promega TNT™ Coupled Wheat Germ Extract Systems 
The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.
Supplier: Promega L4130
Includes: TNT Wheat Germ Extract, Appropriate TNT wheat germ polymerase, Control DNA, TNT Reaction Buffer, Luciferase assay reagent, Luciferase assay wells, Amino acid mixtures
Description
- Single-Tube Coupled Transcription/Translation System
- Produces 2- to 6-fold more protein than standard wheat germ systems
- Produce protein from linearized DNA in 1.5 hours
- You choose the promoter
- U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patents 649289.
- U.S. Patents 5,324,637 and 5,492,817; Australian Patent 660329.
- The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
- Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.
Specifications
| <-65°C | |
| 1mL TNT™ Wheat Germ Extract, 90μL TNT™ Reaction Buffer, 60μL TNT™ RNA Polymerase (SP6, T3 or T7, individually or 2 x 30μL when two Polymerases are | |
| TNT SP6 Coupled Wheat Germ Extract System |
| Translation of mammalian transcription factors | |
| 40 |