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Promega™ TNT™ Coupled Wheat Germ Extract Systems


Ideal for the translation of mammalian transcription factors

Manufacturer: promega™  L4130

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Catalog No. PR-L4130

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Description & Specifications

Specifications

For Use With (Application) Translation of mammalian transcription factors
Includes 1mL TNT™ Wheat Germ Extract, 90μL TNT™ Reaction Buffer, 60μL TNT™ RNA Polymerase (SP6, T3 or T7, individually or 2 x 30μL when two Polymerases are
Product Type TNT SP6 Coupled Wheat Germ Extract System
Quantity 40 Reactions

  • Permit one-tube, coupled transcription/translation reactions for eukaryotic in vitro translation
  • Produce two- to six-fold more protein than standard wheat germ extract translations
  • Eliminate the time and effort required to prepare RNA for a standard wheat germ translation
  • Provide results through autoradiography in six to eight hours
  • Compatible with linear or circular templates (T7 and T3 systems only; SP6 systems should be used with circular DNA only)
  • Available in formats that permit simultaneous expression of multiple genes that are under control of different phage polymerase promoters
  • 40 reactions each