Provide homogeneous, luminescent methods for measuring cytochrome P450 activity
$182.97 - $541.42
U.S. Patent 6,602,677; Australian Pat. No. 754312 and other patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673.
The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit high sensitivity, low background signals and broad dynamic range. The assays employ luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450.
Screen drugs and new chemical entities for their capacity to modulate CYP450 activities in native or recombinant fractions; Measure recombinant CYP450 activities in membrane fractions from heterologous expression systems, such as Sf9 cells and E. coli; and in microsomal fractions from tissues