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Promega P450-Glo™ CYP450 Assay Systems DFS Item

Provide homogeneous, luminescent methods for measuring cytochrome P450 activity

$183.00 - $545.00

Specifications

Detection Method Homogeneous, Luminescent
For Use With (Application) For measuring cytochrome P450 activity
Storage Requirements -20° to -70°C
View More Specs

Includes: Luciferin Detection Reagent; P450-Glo Buffer; 5mM Luciferin-CEE, -ME, -H, -BE, -PFBE, -PPXE, or IPA

Products 19
Catalog Number Mfr. No. Product Type Kit Contents Quantity Price Quantity  
Catalog Number Mfr. No. Product Type Kit Contents Quantity Price Quantity  
PRV8901 View Documents Promega™
V8901
P450-Glo CYP3A4 Assay (Luciferin-PFBE) 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 500μL Luciferin-PPXE 10mL Each for $183.00
PRV8902 View Documents Promega™
V8902
P450-Glo CYP3A4 Assay (Luciferin-PFBE) 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 3 x 500μL Luciferin-PPXE 50mL Each for $545.00
PR-V8792 View Documents Promega™
V8792
P450-Glo CYP2C9 Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 1mL Luciferin-H 5mM 50mL Each for $545.00
PR-V8772 View Documents Promega™
V8772
P450-Glo CYP1A2 Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 1mL Luciferin-ME 5mM 50mL Each for $545.00
PR-V8801 View Documents Promega™
V8801
P450-Glo CYP3A4 Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 100μL Luciferin-BE 10mL Each for $183.00
PR-V8882 View Documents Promega™
V8882
P450-Glo CYP2C19 Screening Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer with Esterase, 2 x 123μg Luciferin-H EGE 50mL Each for $545.00
PR-V8892 View Documents Promega™
V8892
P450-Glo CYP2D6 Screening Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer with Esterase, 1 x 900μg Luciferin-ME EGE 50mL Each for $545.00
PR-V8751 View Documents Promega™
V8751
P450-Glo CYP1A1 Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 70μL Luciferin-CEE 5mM 10mL Each for $183.00
PR-V8752 View Documents Promega™
V8752
P450-Glo CYP1A1 Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 350μL Luciferin-CEE 5mM 50mL Each for $545.00
PR-V8761 View Documents Promega™
V8761
P450-Glo CYP1B1 Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 70μL Luciferin-CEE 5mM 10mL Each for $183.00
PR-V8771 View Documents Promega™
V8771
P450-Glo CYP1A2 Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 200μL Luciferin-ME 5mM 10mL Each for $183.00
PR-V8791 View Documents Promega™
V8791
P450-Glo CYP2C9 Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 200mL Luciferin-H 5mM 10mL Each for $183.00
PR-V8812 View Documents Promega™
V8812
CYP3A7 Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 750μL Luciferin-BE 50mL Each for $545.00
PR-V8762 View Documents Promega™
V8762
P450-Glo CYP1B1 Assay 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 350μL Luciferin-CEE 5mM 50mL Each for $545.00
PR-V8891 View Documents Promega™
V8891
P450-Glo CYP2D6 Screening Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer with Esterase, 1 x 240μg Luciferin-ME EGE 10mL Each for $183.00
PRV9002 View Documents Promega™
V9002
P450-Glo CYP3A4 Assay with Luciferin-IPA 1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer with Esterase, 1 x 60μL Luciferin-IPA 50mL Each for $545.00
PR-V8781 View Documents Promega™
V8781
P450-Glo CYP2C8 Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 300mL Luciferin-ME 5mM 10mL Each for $183.00
PR-V8881 View Documents Promega™
V8881
P450-Glo CYP2C19 Screening Assay 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer with Esterase, 1 x 123μg Luciferin-H EGE 10mL Each for $183.00
PRV9001 View Documents Promega™
V9001*
P450-Glo CYP3A4 Assay with Luciferin-IPA 1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer with esterase, 1 x 15μL Luciferin-IPA 10mL Each for $183.00
Description

Description

The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit high sensitivity, low background signals and broad dynamic range.
The assays employ luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450.

  • Measure the activity of P450s from recombinant or native fractions
  • Test the effects of analytes including drugs and new chemical entities on P450 activities
  • Simple “add-and-read” procedures feature high sensitivity, low background signals, and broad dynamic range
  • Scalable to 384-well format
  • Eliminate interference from fluorescent test compounds
  • Exhibit “glow-type” signal with a half-life of over two hours, eliminating the need for luminometers with injectors and allowing for batch plate processing
  • Formulation reduces the incidence of false positives

  • CYP3A4 Assay with Luciferin-IPA contains a substrate for cytochrome 3A4 that is very well suited for all applications involving human CYP3A4 and is well suited for cell-based applications

Screen drugs and new chemical entities for their capacity to modulate CYP450 activities in native or recombinant fractions; Measure recombinant CYP450 activities in membrane fractions from heterologous expression systems, such as Sf9 cells and E. coli; and in microsomal fractions from tissues

Specifications

Specifications

Homogeneous, Luminescent
For measuring cytochrome P450 activity
-20° to -70°C

U.S. Patent 6,602,677; Australian Pat. No. 754312 and other patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673.