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Promega™ P450-Glo™ CYP450 Assay Systems

Provide homogeneous, luminescent methods for measuring cytochrome P450 activity

$182.97 - $541.42

Specifications

Detection Method Homogeneous, Luminescent
For Use With (Application) For measuring cytochrome P450 activity
Storage Requirements -20° to -70°C
View More Specs
Includes:  Luciferin Detection Reagent; P450-Glo Buffer; 5mM Luciferin-CEE, -ME, -H, -BE, -PFBE, -PPXE, or IPA

 Disclaimers

U.S. Patent 6,602,677; Australian Pat. No. 754312 and other patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673.

Products
Catalog Number Mfr. No. Kit Contents Quantity Product Type Price Quantity    

PRV8901

 
promega™
V8901
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 500μL Luciferin-PPXE 10mL P450-Glo CYP3A4 Assay (Luciferin-PFBE) Each for $182.97

PRV8902

 
promega™
V8902
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 3 x 500μL Luciferin-PPXE 50mL P450-Glo CYP3A4 Assay (Luciferin-PFBE) Each for $541.42

PR-V8792

 
promega™
V8792
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 1mL Luciferin-H 5mM 50mL P450-Glo CYP2C9 Assay Each for $541.42

PR-V8772

 
promega™
V8772
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 1mL Luciferin-ME 5mM 50mL P450-Glo CYP1A2 Assay Each for $541.42

PR-V8801

 
promega™
V8801
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 100μL Luciferin-BE 10mL P450-Glo CYP3A4 Assay Each for $182.97

PR-V8882

 
promega™
V8882
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer with Esterase, 2 x 123μg Luciferin-H EGE 50mL P450-Glo CYP2C19 Screening Assay Each for $541.42

PR-V8892

 
promega™
V8892
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer with Esterase, 1 x 900μg Luciferin-ME EGE 50mL P450-Glo CYP2D6 Screening Assay Each for $541.42

PR-V8751

 
promega™
V8751
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 70μL Luciferin-CEE 5mM 10mL P450-Glo CYP1A1 Assay Each for $182.97

PR-V8752

 
promega™
V8752
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 350μL Luciferin-CEE 5mM 50mL P450-Glo CYP1A1 Assay Each for $541.42

PR-V8761

 
promega™
V8761
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 70μL Luciferin-CEE 5mM 10mL P450-Glo CYP1B1 Assay Each for $182.97

PR-V8771

 
promega™
V8771
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 200μL Luciferin-ME 5mM 10mL P450-Glo CYP1A2 Assay Each for $182.97

PR-V8791

 
promega™
V8791
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 200mL Luciferin-H 5mM 10mL P450-Glo CYP2C9 Assay Each for $182.97

PR-V8812

 
promega™
V8812
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 750μL Luciferin-BE 50mL CYP3A7 Assay Each for $541.42

PR-V8762

 
promega™
V8762
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer, 1 x 350μL Luciferin-CEE 5mM 50mL P450-Glo CYP1B1 Assay Each for $541.42

PR-V8891

 
promega™
V8891
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer with Esterase, 1 x 240μg Luciferin-ME EGE 10mL P450-Glo CYP2D6 Screening Assay Each for $182.97

PRV9002

 
promega™
V9002
1 x 1 Each Luciferin detection reagent, 1 x 50mL Reconstitution Buffer with Esterase, 1 x 60μL Luciferin-IPA 50mL P450-Glo CYP3A4 Assay with Luciferin-IPA Each for $535.00

PR-V8781

 
promega™
V8781
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer, 1 x 300mL Luciferin-ME 5mM 10mL P450-Glo CYP2C8 Assay Each for $182.97

PR-V8881

 
promega™
V8881
1 x 1 Each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer with Esterase, 1 x 123μg Luciferin-H EGE 10mL P450-Glo CYP2C19 Screening Assay Each for $182.97

PR-V8782

 
promega™
V8782
Each for $541.42
Description & Specifications

Specifications

Detection Method Homogeneous, Luminescent
For Use With (Application) For measuring cytochrome P450 activity
Storage Requirements -20° to -70°C

The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit high sensitivity, low background signals and broad dynamic range.
The assays employ luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450.

  • Measure the activity of P450s from recombinant or native fractions
  • Test the effects of analytes including drugs and new chemical entities on P450 activities
  • Simple “add-and-read” procedures feature high sensitivity, low background signals, and broad dynamic range
  • Scalable to 384-well format
  • Eliminate interference from fluorescent test compounds
  • Exhibit “glow-type” signal with a half-life of over two hours, eliminating the need for luminometers with injectors and allowing for batch plate processing
  • Formulation reduces the incidence of false positives

  • CYP3A4 Assay with Luciferin-IPA contains a substrate for cytochrome 3A4 that is very well suited for all applications involving human CYP3A4 and is well suited for cell-based applications

Screen drugs and new chemical entities for their capacity to modulate CYP450 activities in native or recombinant fractions; Measure recombinant CYP450 activities in membrane fractions from heterologous expression systems, such as Sf9 cells and E. coli; and in microsomal fractions from tissues