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Promega™ CellTox™ Green Cytotoxicity Assay


Ideal for determining toxic effects during or at endpoint of extended exposure treatment procedures

Manufacturer: promega™  G1821

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Catalog No. PRG1821

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Description & Specifications

Specifications

Quantity 5mL
For Use With (Application) Ideal for determining toxic effects during or at endpoint of extended exposure treatment procedures
Storage Requirements -20°C

Assay system measures changes in membrane integrity that occur as result of cell death. It is intended to assess cytotoxicity in cell culture after experimental manipulation. It uses proprietry asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells. When dye binds DNA released from cells, its fluorescence properties are substantially enhanced. Viable cells produce no appreciable increases in fluorescence. Therefore, fluorescence signal produced by binding interaction with dead cell DNA is proportional to cytotoxicity.

  • Binds DNA of cells that have lost membrane integrity throughout 72-hour exposure
  • Dye can be diluted in culture medium and delivered directly to cells at seeding or at dosing or diluted in assay buffer and delivered to cells as endpoint measure after exposure period
  • Can scale from 96- to 1536-well plate formats with “no-addition” or “single-addition” protocols
  • Available as CellTox Green Cytotoxicity Assay and CellTox Green Express Cytotoxicity Assay kits
  • Lysis solution also available separately
CellTox Green Cytotoxicity Assay
  • 100μL/assay in 96-well plate or 25μL/assay in 384-well plate format
  • Follows Endpoint Assay Protocol, 2X reagent addition
  • Kit contains green dye, assay buffer and lysis solution
CellTox Green Express Cytotoxicity Assay
  • For 96-well plate or 384-well plate format when used at recommended final dilution of 1:1,000 in assay well
  • Includes 200μL Green dye