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Promega™ CellTiter-Fluor™ Cell Viability Assay DFS Item

Nonlytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population based on measurement of a conserved and constitutive protease activity within live cells

$161.57 - $1,048.60


Detection Method Fluorescence
For Use With (Application) For normalizing data for live cell number
For Use With (Equipment) 96- or 384-well plates
Storage Requirements -20°C
View More Specs


Patent Pending.

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
PRG6080 Promega™
10mL Each for $161.57
PRG6081 Promega™
5 x 10mL Each for $683.73
PRG6082 Promega™
2 x 50mL Each for $1,048.60


The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.

  • Can be performed in multiplex with many Promega luminescence assays or spectrally distinct fluorescence assays, eliminating parallel plate processing and reducing cell culture costs
  • Ideal for normalizing data for live cell number, making results more comparable well-to-well, plate-to-plate, and day-to-day

Description & Specifications


For normalizing data for live cell number
96- or 384-well plates