Promega™ CellTiter-Fluor™ Cell Viability Assay
Nonlytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population based on measurement of a conserved and constitutive protease activity within live cells
$161.57 - $1,048.60
The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.
- Can be performed in multiplex with many Promega luminescence assays or spectrally distinct fluorescence assays, eliminating parallel plate processing and reducing cell culture costs
- Ideal for normalizing data for live cell number, making results more comparable well-to-well, plate-to-plate, and day-to-day
|For normalizing data for live cell number|
|96- or 384-well plates|