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Promega™ Caspase-Glo™ 3/7 Assay Kit

Provides a homogeneous luminescent assay that measures Caspase-3/7 activities

$175.48 - $4,378.44

Specifications

For Use With (Application) 96- or 384-well plates using either purified Enzyme or cells in culture as sample
Storage Requirements -20°C
View More Specs
Includes:  Caspase-Glo 3/7 Substrate; Caspase-Glo 3/7 Buffer
Products
Catalog Number Mfr. No. Quantity Price Quantity    

PR-G8091

 
promega™
G8091
10mL Each for $575.66

PR-G8092

 
promega™
G8092
100mL Each for $2,943.57

PR-G8090

 
promega™
G8090
2.5mL Each for $175.48

PR-G8093

 
promega™
G8093
10 x 10mL Each for $4,378.44
Description & Specifications

Specifications

For Use With (Application) 96- or 384-well plates using either purified Enzyme or cells in culture as sample
Storage Requirements -20°C

Utilizes a proluminescent Caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a single reagent optimized for Caspase-3/7 activity, luciferase activity, and cell lysis. Stabilized luciferase improves assay performance across a wide range of assay conditions and makes the assay less likely to be affected by compound interference than other luminescent-, fluorescent-, or colorimetric-based assays.

  • Designed for use with 96- or 384-well plates using either purified enzyme or cells in culture as sample
  • Requires less enzyme or fewer cells than other caspase formats due to low background luminescence
  • Requires no sample preparation, manipulation, or extended incubation times—maximum sensitivity is achieved in as little as one-half to one hour
  • “Add-mix-measure” protocol makes the assay easy to automate
  • Extended-glow signal allows the plates to be read over a three-hour period for batch processing—no injectors required

Patent pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patent Nos. 5,583,024; 5,674,713; and 5,700,673.