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  6. Promega AMV Reverse Transcriptase

Promega AMV Reverse Transcriptase

Catalog No. PRM9004
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Description:
AMV Reverse Transcriptase
AMV Reverse Transcriptase (HC)
Quantity:
300U
600U
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Catalog No. Description Quantity
PRM9004 AMV Reverse Transcriptase (HC) 600U
PRM5101 AMV Reverse Transcriptase 300U
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Catalog No. PRM9004 Supplier Promega Supplier No. M9004
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Description

Includes

5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT)

AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

  • Efficient Reverse Transcription of Templates with High Secondary Structure
  • DNA polymerization using template DNA, RNA or RNA:DNA hybrids
  • Requires a primer as well as magnesium or manganese
  • Stable at higher reaction temperatures (37-58°C)
  • Supplied at a concentration of 10u/μL (Cat.# M5101, M5108) or 20-25u/μL (Cat.# M9004)

Specifications

Concentration 20 to 25 U/μL
Content And Storage −30 to −10°C
Description AMV Reverse Transcriptase (HC)
Form Liquid
Quantity 600U
For Use With (Application) cDNA Synthesis
Includes 1 x 500μL 5X reaction buffer
Source Avian Myeloblastosis Virus
Storage Buffer Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50%