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Invitrogen™ ProLong™ Gold Antifade Mountant with DNA Stain DAPI
Description
ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image. ProLong Gold Antifade Mountants come in a 2-mL dropper bottle or 10-mL bottle. It is formulated with the blue DNA stain DAPI to make your nuclear counterstaining more convenient and consistent when preparing your samples for imaging.
ProLong Gold Antifade Mountant with DNA Stains DAPI contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. It is a curing mounting media that forms an optical path of 1.47 RI and allows longer-term storage of the sample.
Many mountants can quench initial fluorescence signal strength by up to 80%. ProLong Gold Antifade Mountant was developed to minimize this initial signal quenching for the broadest range of colors and dyes and is formulated with the DAPI DNA nuclear stain to simplify your experiments. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter.
ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP but can be used with Alexa Fluor dyes of your choice. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. ProLong Glass mountants are recommended for all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant with DAPI.
Specifications
Specifications
| Quantity | 1 x 2 mL |
| Product Type | Antifade Mountant |
| Content And Storage | Store at room temperature. |
| Product Line | ProLong |
| Reagent Type | Mounting Solution, Antifade Solution |
| Volume (Metric) | 2 mL |
Frequently Asked Questions (FAQs)
They are composed of proprietary antifades and polymers in aqueous buffers.
No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.
Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').
We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.
No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.