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Gibco™ Primary Rat Hippocampal Neurons, Sprague Dawley

Catalog No. A36513
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Catalog No. A36513 Supplier Gibco™ Supplier No. A36513
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Description

Gibco Primary Rat Hippocampal Neurons, Sprague Dawley, are isolated and cryopreserved from Sprague Dawley embryonic day-18 rats.

A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains one million cells per mL. These neurons consistently yield post-thaw viability in the range of 50–80%, have high neuronal purity, and are validated for functionality.

• High viability: post-thaw viability of 50% or greater
• High purity: greater than 90% neuronal phenotype
• Functionally validated: tested for spontaneous synchronous calcium flux and synaptic release activity

Specifications

Content And Storage Store in liquid nitrogen, vapor-phase.
Age Fetal
Cell Type Neural Cells
Culture Type Mammalian Cell Culture
Form Cryopreserved
Species Rat
Tissue Hippocampus
No. of Cells 1 x 106
Product Type Rat Hippocampus Neuron
Quantity 1 x 106 cells
Tested For Cell Purity, Sterility, Viability, Mycoplasma
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Frequently Asked Questions (FAQs)

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.
What is the purpose of L-glutamate in the media for primary hippocampal neurons?

Glutamate helps hippocampal neurons sprout and promotes viability. However, L-glutamate can only be supplemented in the medium up to day 4 in culture because it is toxic to mature neurons.
Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.

Why does the manual for primary rat neurons have the instruction "Do not expose neurons to air at any time"?

Neurons tend to lift off from the complete medium change, which exposes cells to air. By changing half of the medium, the disturbance can be minimized.

For Research Use Only. Not for use in diagnostic procedures.