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Thermo Scientific™ PolarScreen™ ER Alpha Competitor Assay, Green
Description
- The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands. The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat. No. A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone ES2 Green) in a homogenous, mix-and-read assay format.
- This kit produces a green color read-out; a red kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation.
- Complete & ready-to-use out of the box - all reagents included, just add your test compounds
- High throughput compatible - 800 x 20 μL fluorescence polarization assays in a 384-well plate
- Convenient - available in bulk sizes for larger screening needs
- Includes:
- 2 tubes full-length ER Alpha Recombinant Protein (store at -68 to -85°C)
- 1 tube of Fluormone ES2 Green Tracer (store at -5 to -30°C)
- 1 bottle of ES2 Screening Buffer (store at -5 to -30°C)
Specifications
Specifications
| Content And Storage | Contains: • 2 tubes full-length ER Alpha Recombinant Protein (store at -68 to -85°C) • 1 tube of Fluormone ES2 Green Tracer (store at -5 to -30°C) • 1 bottle of ES2 Screening Buffer (store at -5 to -30°C) |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Fluorescent Imager |
| Format | 384-well Plate |
| Quantity | 800 Assays |
| Type | ER Alpha Competitor Assay |
Frequently Asked Questions (FAQs)
We highly recommend that you use the template file because it contains all of the necessary experimental details for your qPCR run. If the template file is not working for you, please send an email to our stem cell technical support specialists at: stemcell-help@thermofisher.com.
-No assay window: Check that your instrument is designed to do FP experiments.
-Poor discrimination between the minimum and maximum mP Control (both include the receptor): This may be an indication of the quality of the receptor. Do not make single use aliquots of the receptor or store diluted.
-The type of microplate is critical: Please see if the protocol specifies untreated polystyrene or NBS-coated plates. If the plate binds the fluorescent substrate, it cannot freely rotate and the mP reading will always be high. White plates cannot be used for FP assays. Avoid the use of silanized pipette tips and vials.
-The “No Receptor Control' (free Fluormone/Tracer) should have much higher RFU values compared to buffer alone. If not, there is a problem with the instrument setup or less commonly with the Fluormone/Tracer.
-If the free Fluormone/Tracer mP value is greater than 100 mP or negative, please refer to the user manual for your instrument and reset the G factor.
For FP assays, plate readers must specifically have FP built in. Please see our instrument compatibility portal (http://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/drug-discovery-development/dd-misc/instrument-compatibility-portal.html).
We offer a FP One-Step Reference Kit (https://www.thermofisher.com/order/catalog/product/P3088) to detect in green (Ex/Em = 488/535 nm) and red (Ex/Em = 525/590 nm) emission ranges, suitable for the PolarScreen Green and Red Assays. The alternative is to perform the Controls in the respective assay kit prior to performing the actual assay using test compounds.
For Research Use Only. Not for use in diagnostic procedures.