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Thermo Scientific™ Pierce™ SuperSignal West Femto Fast Western Blot Kit

For mid-to-low femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Manufacturer:  Thermo Scientific™ 35080

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Catalog No. PI35080



Thermo Scientific™ Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol that allows mid to low femtogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrates sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.


  • Fast – low femtogram levels of detection in about 1 hour

  • Convenient – no expensive hardware or vacuum required; no clogging issues

  • Simple – optimized protocol makes Western blot analysis easier than ever

  • Economical – no additional consumables or extra equipment to purchase

  • Stable – all kit components are stable for one year stored at 4°C

  • Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody

Compatible with:

Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer



Fast Western Antibody Diluent, 200mL, Fast Western Mouse Optimized HRP Reagent, Femto, 10mL, SuperSignal West Femto Luminol/Enhancer Solution, 50mL, SuperSignal West Stable Peroxide Solution, 50mL, Fast Western 10X Wash Buffer, 100mL
10 mini blots probed with mouse primary antibodies
Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate, Mouse
Multi-component kit
400mL kit