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Thermo Scientific™ Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Rabbit

Catalog No. PI35071
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400 mL
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PI35071 400 mL
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Catalog No. PI35071 Supplier Thermo Scientific™ Supplier No. 35071
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Description

For femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate contains optimized reagents and protocol that allows femtogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a chemiluminescent signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

Highlights:

  • Fast – femtogram levels of detection in about one hour

  • Convenient – no expensive hardware or vacuum required; no clogging issues

  • Simple – optimized protocol makes Western blot analysis easier than ever

  • Economical – no additional consumables or extra equipment to purchase

  • Stable – all kit components are stable for one year stored at 4°C

  • Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody

Compatible with:

Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer

Specifications

Description Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate, Rabbit
Product Type Western Blot Kit
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce, SuperSignal
Quantity 400 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Rabbit
Sensitivity Femtogram-level
Sufficient For 10 western blots (800 sq. cm of membrane)
Content And Storage Sufficient For: 10 mini blots probed with rabbit primary antibodies
• Fast Western Antibody Diluent, 200 mL
• Fast Western 10X Wash Buffer, 100 mL
• Fast Western Rabbit Optimized HRP Reagent, Dura, 10 mL
• SuperSignal West Dura Luminol/Enhancer Solution, 50 mL
• SuperSignal West Stable Peroxide Solution, 50 mL

Store kit at 4°C.
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Frequently Asked Questions (FAQs)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

For Research Use Only. Not for use in diagnostic procedures.