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Thermo Scientific™ Pierce™ SDS-PAGE Sample Prep Kit

Catalog No. PI89888
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50 reactions
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PI89888 50 reactions
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Catalog No. PI89888 Supplier Thermo Scientific™ Supplier No. 89888
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Description

Quickly and easily remove high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis.

Thermo Scientific™ Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Highlights:

  • Eliminates gel artifacts caused by incompatible contaminants – removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis

  • Compatible with the BCA protein assay – allows protein quantitation of the processed sample before gel-loading

  • Enriches dilute protein solutions – concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes

  • Powerful and flexible – process 2 to 350μL of sample containing up to 70μg of protein; recover 75 to 85% of the total protein in 50μL of elution buffer

  • Convenient, complete kit – includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

    • Includes:

      PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

Specifications

Content And Storage Upon receipt store kit at room temperature.
Buffer Sample Loading Buffers
Gel Type SDS-PAGE
Gel Compatibility SDS-PAGE Gels
Quantity 50 reactions
Product Line Pierce
Product Type SDS-PAGE Sample Prep Kit

For Research Use Only. Not for use in diagnostic procedures.