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Thermo Scientific™ Pierce™ Recombinant Protein L and Conjugates

Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.

Manufacturer:  Thermo Scientific™ PI32420

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Catalog No. PI32420



Description

Description

Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein L enable probing and detection of mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Highlights:

  • Contains four immunoglobulin-binding domains per protein
  • Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
  • Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains
Specifications

Specifications

Pierce Recombinant Protein L, Peroxidase Conjugated
Lyophilized powder from protein in buffered solution
0.5mg
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