missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Antibody-Binding Proteins and Conjugates
  5. Thermo Scientific™ Pierce™ Recombinant Protein G

Thermo Scientific™ Pierce™ Recombinant Protein G

Catalog No. PI21193
Change view
Click to view available options
Quantity:
5 mg
50 mg
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI21193 5 mg
PI77675 50 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. PI21193 Supplier Thermo Scientific™ Supplier No. 21193
Only null left

Description

Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA

Specifications

Product Type Protein G
Quantity 5 mg
Product Line Pierce
Form Powder
Protein Subtype Protein G
Target Antibodies
Content And Storage Upon receipt store at -20°C. Product is shipped at ambient temperature.

For Research Use Only. Not for use in diagnostic procedures.