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Thermo Scientific™ Pierce™ Recombinant Protein G and Conjugates


Detect mouse and human antibodies, especially IgG isotypes, in immunoassays with this purified, biotinylated or peroxidase-conjugated recombinant Protein G.

Manufacturer: thermo scientific™  PI29988

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Catalog No. PI29988

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Description & Specifications

Specifications

Description Pierce Recombinant Protein G, Biotinylated
Formulation Lyophilized powder from protein in salt-free solution
Quantity 0.5mg

Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Protein G is a bacterial cell wall protein isolated from group G Streptococci. DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2.

Highlights:

  • Contains two Fc-binding domains per protein
  • Better than Protein A for mouse (incl. IgG1), human (incl. IgG3) and rat, goat and cow antibodies
  • Poorer than Protein A for guinea pig, pig, dog and cat
  • Does not bind human IgM, IgD or IgA