Thermo Scientific™ Pierce™ Recombinant Protein A/G and Conjugates
Detect antibodies, especially IgG isotypes, from many different species hosts with this purified, alkaline phosphatase- or peroxidase-conjugated recombinant Protein A/G.
Manufacturer: Thermo Scientific™ PI32391
Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein A/G enable probing and detection of antibodies, especially IgG isotypes, from many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin. Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G.
- Contains four Fc-binding domains from Protein A and two from Protein G
- Provides binding for all antibody species and subclasses recognized by either Protein A or Protein G
|Pierce Recombinant Protein A, Alkaline Phosphatase Conjugated|
|Approx. 0.5mg/mL in buffered saline solution and 50% glycerol|