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Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein A enable probing and detection of rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. coli and functions essentially the same as native Protein A. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6).
- Contains four Fc-binding domains per protein
- Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
- Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies