Thermo Scientific™ Pierce™ Protein Refolding Kit

The kit contains the essential reagents and complete strategy for determining optimal buffer conditions to refold denatured recombinant proteins to restore native structure and function. Nine base refolding buffers form a matrix that includes a range of strong and weak denaturant conditions for the suppression of protein aggregation. The supplied additives are used as additional matrix factors, depending on the protein type being refolded. Buffer components are examined at three concentration levels, allowing a wide spectrum of folding conditions to be tested within one experiment. The adjustable design allows matrix conditions to be tailored to the target protein, preventing sample waste and unnecessary analysis, while maximizing refolding yields. The Pierce Protein Refolding Kit is accompanied by a comprehensive Refolding Guide with details on isolating, solubilizing and purifying inclusion bodies; optimizing refolding conditions; and analyzing refolding yields.

Highlights:

• Robust – conditions and components examined are limited to those having the most significant and general utility as folding buffers

• Convenient – three-level matrix design significantly reduces the amount of secondary optimization required and increases the ease of data interpretation

• Adjustable matrix format – allows refolding experiments to be customized to the target protein; known positive and negative interactions between buffer components are addressed, minimizing unnecessary analyses

• High-purity reagents – reagents are formulated using stringent standards so that consistent results are attained

Includes:

Nine different Base Refolding Buffers (10mL each), convenient amounts of seven additives, and a comprehensive Refolding Guidebook

Compatible with:

Thermo Scientific Inclusion Body Solubilization Reagent (Part No. 78115)