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Thermo Scientific™ PageBlue™ Protein Staining Solution

A sensitive formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Manufacturer:  Thermo Scientific™ 0024620

Catalog No. PI24620



Description

Description

Thermo Scientific Pierce PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

The simple PageBlue Protein Staining Solution protocol is sensitive, efficient and eliminates the concern of over staining gels even after overnight staining. This protein stain delivers a dynamic range of 5 to 500ng, which is approximately 10 times more sensitive than traditional coomassie R-250-based dyes. Furthermore, PageBlue Protein Staining Solution is economical because it can be reused up to three times without any loss in sensitivity.

Highlights:

  • Easy-to-use – simply soak the gel in the ready-to-use solution and observe stained bands without destaining

  • Fast – 25 to 40 minute protocol

  • Safe – does not contain methanol or acetic acid

  • Dynamic range – linear dynamic range of 5 to 500ng

  • Economical – can be reused up to 3 times

Recommended for:

Visualize and quantify proteins separated in 1D, 2D and IEF polyacrylamide gels; Stain proteins after transfer onto PVDF membranes; Stain gels prior to subsequent silver staining or mass spectrometry analysis

Specifications

Specifications

In-Blot Detection, In-Gel Detection
PageBlue™
Protein Staining Solution
Protein
Colorimetric
Coomassie
SDS
Documents
Product Certifications