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Thermo Scientific™ PageBlue™ Protein Staining Solution

Catalog No. PI24620
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Catalog No. PI24620 Supplier Thermo Scientific™ Supplier No. 24620
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Description

A sensitive formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Thermo Scientific Pierce PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid. This protein stain delivers a dynamic range of 5 to 500 ng, which is approximately 10 times more sensitive than traditional coomassie R-250-based dyes. Furthermore, PageBlue Protein Staining Solution is economical because it can be reused up to three times without any loss in sensitivity.

Highlights:

  • Easy-to-use – simply soak the gel in the ready-to-use solution and observe stained bands without destaining
  • Fast – 25 to 40 minute protocol
  • Safe – does not contain methanol or acetic acid
  • Dynamic range – linear dynamic range of 5 to 500 ng
  • Economical – can be reused up to 3 times

Recommended for:

  • Visualize and quantify proteins separated in 1D, 2D and IEF polyacrylamide gels
  • Stain proteins after transfer onto PVDF membranes
  • Stain gels prior to subsequent silver staining or mass spectrometry analysis

Specifications

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature
Detection Location In-Blot Detection, In-Gel Detection
Detection Method Colorimetric
Label or Dye Coomassie
Quantity 1 L
Target Molecule Protein
Product Line PageBlue
Product Type Protein Staining Solution

Frequently Asked Questions (FAQs)

After staining my PVDF membrane with PageBlue Protein Staining Solution, I am getting high background on the membrane. Can you offer some tips?

This is most likely due to insufficient destaining time. We recommend destaining the membrane in 30% acetonitrile/20% ethanol solution for an additional 5 mins.
After staining my PVDF membrane with PageBlue Protein Staining Solution, I am not seeing any band development. What went wrong?

This is most likely due to a problem with the western transfer. Please confirm that the transfer buffer and transfer conditions are correct.
After staining my protein gel with PageBlue Protein Staining Solution, I am not seeing any band development. What went wrong?

Here are possible causes and solutions:

- No protein was present in sample. Load a known amount of purified protein as a control.
- Insufficient amount of protein in sample. Load more total protein in gel.
- SDS not completely removed from gel. Wash gel more extensively before staining.
I stained my protein gel with Thermo Scientific PageBlue Protein Staining Solution and am getting very high background. Do you have any tips?

This is most likely due to SDS interference. We recommend increasing the number of washes and/or wash volumes before staining. Destaining the gel for 5 minutes with 25% isopropanol/10% acetic acid solution or 12% trichloroacetic acid will also help.
Do you have any special recommendations for staining small proteins (less than 10 kDa) with the PageBlue Protein Staining Solution?

Small proteins (less than 10 kDa) are susceptible to leaching from the gel during the staining procedure and require fixation with glutaraldehyde before staining the gel with PageBlue Protein Staining Solution. Other common protein fixatives (e.g., acetic acid, isopropanol, ethanol, TCA, etc.) are not suitable for this purpose, as proteins will be washed out of the gel during the staining procedure. Please see procedure for fixation, mentioned on Page 3 of the manual.
I am planning to use the PageBlue Protein Staining Solution to stain native gels. Should I make any changes to the procedure?

The first wash step in the staining procedure is designed to remove sodium dodecyl sulfate (SDS) from the gel, because SDS interferes with staining. Native gels do not contain SDS and, therefore, this step can be omitted from native PAGE applications.
Do you have any suggestions for increasing sensitivity with the PageBlue Protein Staining Solution?

Fixing gel proteins with 25% isopropanol/10% acetic acid solution or 12% trichloroacetic acid (TCA) for 15 minutes can increase staining sensitivity.
What is the sensitivity of the PageBlue Protein Staining Solution?

It can detect proteins with a dynamic range of 5-500 ng, which is approximately 10 times more sensitive than traditional Coomassie R-250-based dyes.
Can I reuse the PageBlue Protein Staining Solution?

The PageBlue Protein Staining Solution can be reused up to three times without noticeable loss in detection sensitivity.
Does the PageBlue Protein Staining Solution contain Coomassie G-250 or Coomassie R-250?

PageBlue Protein Staining Solution contains Coomassie G-250 for protein staining on polyacrylamide gels and PVDF membranes. The rest of the composition is proprietary.
How should I store the Thermo Scientific PageBlue Protein Staining Solution and what is its shelf life?

We recommend storing the PageBlue Protein Staining Solution at room temperature where it is stable for a year.

For Research Use Only. Not for use in diagnostic procedures.