Thermo Scientific™ Pierce Formaldehyde Ampules are vials of high-quality, methanol-free formaldehyde for use as a general immunohistochemistry fixative and reversible amine-crosslinking agent for protein and nucleic acid tissue samples.Formaldehyde is a highly reactive, cell-permeable agent that is used as a reversible cross-linking agent for proteins and nucleic acids within the cell or as a general cell-fixing agent for imaging-based applications. Cell permeability allows the reagent to readily enter living cells, enabling intracellular applications, such as protein interaction discovery or bioimaging. Formaldehyde produces reversible cross-links with protein and nucleic acids by coupling neighboring primary amines. Cross-links can be reversed simply by heating the sample.
Pure and stable - prepared directly from pure granular paraformaldehyde and sealed in ampules under an inert atmosphere, ensuring that it is methanol-free and resistant to oxidation
Versatile - use to cross-link molecules in protein-protein and protein-nucleic acid interaction discovery, bioimaging and flow cytometry
Aliquot packaging - choose 1mL or 10mL ampules suitable for low- and high-volume applications ranging from culture-targeted cell fixing to in-cell protein cross-linking
Convenient starting concentration - 16% (w/v) formaldehyde solution in ultrapure water simplifies dilution to the required working concentration