Thermo Scientific™ Pierce™ LDS Sample Buffer, Non-Reducing (4X)

Description

Thermo Scientific™ Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer optimized for use with Pierce Protein SDS-PAGE Gels.

LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both Coomassie and silver staining and Western blotting applications.

Highlights:

• Concentrated – 4X formulation provides more versatility than traditional 2X buffers

• Nonreducing – ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions

• SDS alternative – lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well

• Physiologic pH – buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)

• Lane marker dyes – includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front

• Convenient – stable at room temperature, enabling storage at the bench where electrophoresis is performed