Thermo Scientific™ Pierce™ Glycoprotein Staining Kit

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

• Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups

• Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)

• Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background

• Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band

• Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).

• Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes

Includes:

Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)