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Thermo Scientific™ Pierce™ Glycoprotein Staining Kit
Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.
Supplier: Thermo Scientific™ 24562
Description
Thermo Scientific™ Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.
The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.Highlights:
Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background
Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band
Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).
Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes
Includes:
Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)
Specifications
| Sufficient For: 10 mini gels (SDS-PAGE) • Oxidation Reagent, 2.5 g powder to make 250 mL • Reduction Reagent, 1.25 g powder to make 250 mL • Positive Control (Horseradish Peroxidase), 1 mg • Negative Control (Soybean Trypsin Inhibitor), 1 mg • Glycoprotein Stain, 250 mL Store all components at 4°C. |
|
| Colorimetric | |
| Glycoprotein Staining Kit | |
| 750 mL |
| In-Blot Detection, In-Gel Detection | |
| Fuchsin Sulfite | |
| Pierce | |
| Proteins (Glycoproteins) |
For Research Use Only. Not for use in diagnostic procedures.