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Thermo Scientific™ Pierce™ Glutathione Coated Plates
Description
Pierce Glutathione Coated Plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.
Features of Glutathione Coated Plates include:
• Selective—specifically binds fusion proteins containing glutathione S-transferase
• Capacity—binds approx. 10 ng GST protein per well
• Versatile—ready-to-bind plate format is useful for protein interaction studies
• Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
• Ready to use—Pre-blocked with SuperBlock Blocking Buffer to reduce nonspecific binding
Specifications
Specifications
| Binding Property | Standard Capacity, Affinity |
| Format | Standard |
| Content And Storage | Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag with desiccant at 4°C. |
| Detection Method | Fluorescence |
| Packaging | 5 Plates |
| Product Line | Pierce |
| Color | Black |
| Product Type | Microplate |
| Material | Polystyrene |
| No. of Wells | 96 |
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Frequently Asked Questions (FAQs)
The background is extremely low when using the Pierce Glutathione Coated Plates. This results in a high signal-to-noise ratio and a low detection limit which in general means that the detection limit (OD 0.5) is 3 ng per well (100 µL).
The Pierce Glutathione Coated Plates are extremely stable over long periods of time, if stored at room temperature (20-25 degrees C). We guarantee 12 mths of shelf life in unopened foil package after the date of shipment.
To determine the approximate concentration of a GST-tagged protein, a standard curve should be generated using purified GST added in different concentrations to the wells of the plates. Please note that it is only the approximately concentration that can be determined since not 100% of the GST-fusion proteins will be bound due to denaturing or steric hindrance to GSH.
No. Do not whirl-mix a solution containing GST-tagged proteins. The CV will increase due to slight degradation of the GST protein.
We recommend using 0.05% Tween-20 surfactant (as the non-ionic detergent) to improve the signal-to-noise ratio of the assay and thus decrease the background.