Thermo Scientific™ Pierce™ Glutathione Coated Plates

Description

Pierce Glutathione Coated Plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.

Thermo Scientific Pierce Glutathione Coated Plates are microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of Glutathione Coated Plates include:
• Selective—specifically binds fusion proteins containing glutathione S-transferase
• Capacity—binds approx. 10 ng GST protein per well
• Versatile—ready-to-bind plate format is useful for protein interaction studies
• Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
• Ready to use—Pre-blocked with SuperBlock Blocking Buffer to reduce nonspecific binding