missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. Target-Specific Western Blot Kits
  5. Thermo Scientific™ Pierce™ Fast Western Kit, SuperSignal™ West Pico, Mouse

Thermo Scientific™ Pierce™ Fast Western Kit, SuperSignal™ West Pico, Mouse

Catalog No. PI35060
Change view
Click to view available options
Quantity:
800 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI35060 800 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI35060 Supplier Thermo Scientific™ Supplier No. LSG35060
Only null left

Description

For picogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol that allows picogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.

These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a chemiluminescent signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.

Highlights:

  • Fast – picogram levels of detection in about one hour
  • Convenient – no expensive hardware or vacuum required; no clogging issues
  • Simple – optimized protocol makes Western blot analysis easier than ever
  • Economical – no additional consumables or extra equipment to purchase
  • Stable – all kit components are stable for one year stored at 4°C
  • Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody

Compatible with:

Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer

Specifications

Product Type Western Blot Kit
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce, SuperSignal
Quantity 800 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Mouse
Sensitivity Picogram-level
Sufficient For 20 Mini Blots
Content And Storage Sufficient For: 20 mini blots probed with mouse primary antibodies
• Fast Western Antibody Diluent, 400 mL
• Fast Western 10X Wash Buffer, 200 mL
• Fast Western Mouse Optimized HRP Reagent, Pico, 20 mL
• SuperSignal West Pico Luminol/Enhancer Solution, 100 mL
• SuperSignal West Stable Peroxide Solution, 100 mL

Store kit at 4°C.
Show More Show Less

Frequently Asked Questions (FAQs)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

For Research Use Only. Not for use in diagnostic procedures.