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Thermo Scientific™ Ethidium Bromide Solution (0.625 mg/mL)

Catalog No. PI17896
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PI17896 5 mL
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Catalog No. PI17896 Supplier Thermo Scientific™ Supplier No. LSG17896
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Description

Visualize DNA fluorescently in a variety of molecular biology applications using these dilute (0.625mg/mL) or concentrated (10mg/mL) ethidium bromide solutions.

Thermo Scientific™ Pierce Ethidium Bromide is available as both dilute (0.625mg/mL) and more concentrated (10mg/mL) solutions to accommodate most molecular biology applications for fluorescent DNA visualization.

Thermo Scientific™ Pierce Ethidium Bromide is available as both dilute (0.625mg/mL) and more concentrated (10mg/mL) solutions to accommodate most molecular biology applications for fluorescent DNA visualization.

Highlights:

0.625mg/mL EtBr Solution:

  • Each drop contains 25μg of ethidium bromide and is ideal for a 50mL agarose gel
  • After adding one drop of solution per 50mL of gel, the final concentration of EtBr will be 0.5μg/mL, which is the recommended concentration for electrophoresis of nucleic acids
  • Can also be used as the running buffer or for staining gels after electrophoresis

10mg/mL EtBr Solution:

  • A safe and convenient way to use ethidium bromide while minimizing exposure

Specifications

Content And Storage Store at room temperature, protected from light.
Detection Location In-Gel Detection
Detection Method Fluorescence
Label or Dye Ethidium Bromide
Quantity 5 mL
Target Molecule RNA, DNA
Product Type Ethidium Bromide Solution

Frequently Asked Questions (FAQs)

Do you have a protocol for ethidium bromide staining in agarose gels?

For use in agarose gels:

- Add ethidium bromide to melted agarose to a final concentration of 0.5 µg/mL. Do not melt agarose that already contains ethidium bromide.

For staining agarose gels after electrophoresis:

- You can stain gels that have been run in the absence of ethidium bromide by covering the gel in 0.5 µg/mL ethidium bromide in water and gently agitating for 10 to 30 minutes.
- If necessary, gels can be destained by shaking in H2O for an additional 30 minutes. To stain RNA gels, you should minimize staining time and definitely include a destaining period.

What can ethidium bromide be used to detect and what is its sensitivity?

Ethidium bromide can be used to detect ssDNA, RNA, and dsDNA. This fluorescent dye intercalates between the stacked bases of nucleic acids, and exhibits an increased fluorescence at 590 nm. Ethidium bromide can detect down to ~1-10 ng/band of dsDNA in an agarose gel.

For Research Use Only. Not for use in diagnostic procedures.