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Applied Biosystems™ Pharma KingFisher™ Flex 96 Standard Plates

Catalog No. A43076
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A43076 48 Plates
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Catalog No. A43076 Supplier Applied Biosystems™ Supplier No. A43076

Description

These standard plates are for use with the Pharma KingFisher Flex 96 Deep-Well Head Magnetic Particle Processor or MagMAX Express-96 Standard Magnetic Particle Processor in combination with PrepSEQ ResDNA kits and ProteinSEQ kits.

These standard plates are for use with the Pharma KingFisher Flex 96 Deep-Well Head Magnetic Particle Processor or MagMAX Express-96 Standard Magnetic Particle Processor in combination with PrepSEQ ResDNA kits and ProteinSEQ kits. The standard configuration differs from the deep-well configuration in that the volume of sample input is from 20–200 μL per well compared to 100–1000 μL per well for the deep-well configuration.

Optimize performance using KingFisher Flex System consumables designed specifically for use with these instruments. KingFisher Flex disposable plastics (tip combs and microplates) are made of polypropylene and are ideal for magnetic particle processing because of their low binding affinity for biomolecules. Their special design enables excellent recovery of magnetic beads.

Specifications

Barcode No Barcode
Content And Storage 48 standard-well 96-well plates
Packaging 48 Plates
Product Line KingFisher Flex
Color Clear
Product Type Microplate
For Use With (Equipment) KingFisher™ Flex
Material Polypropylene
Volume (Metric) Well 1000 μL
No. of Wells 96
Quantity 48 Plates
Shipping Condition Room Temperature
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Frequently Asked Questions (FAQs)

My Pharma KingFisher Flex run was stopped due to an error message. Can I start the protocol from where it stopped?

No, once a protocol was stopped, it will restart from the beginning. The extraction procedure can either be finished manually by using a magnetic stand, or a modified version of the original Pharma KingFisher Flex script would have to be created that starts off at the point where the run was stopped.

Is it always necessary to use the transport locks on the Pharma KingFisher Flex instrument?

The transport locks are only necessary when relocating the instrument.
Are the volumes of reagents in each well critical for a successful Pharma KingFisher Flex run?

We strongly recommend that you maintain the specified volumes within the defined limits to avoid spillover and to help ensure the best performance at the most efficient level.
What if the magnetic particles are attached to the tip combs after my Pharma KingFisher Flex run?

This happens sometimes but it will not affect the yield because the sample has been released from the particles.
What if the magnetic particles remain in the sample well during a Pharma KingFisher Flex run?

If the starting material is too viscous, the magnetic rods will not be able to collect the particles. Dilute the sample and check that the sample is properly homogenized/lysed. Adding low amounts of detergent will improve the collection of magnetic particles as well. Quickly centrifuge the plate to sediment the particles to the bottom of the plate.
What should I do if I forget to insert the tip combs into the tip comb holder for the Pharma KingFisher Flex instrument?

The protocol will not start without the tip combs inserted into the tip comb holder.
What should I do if the Pharma KingFisher Flex magnet sets are dirty?

Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution, soap solution, or alcohol.
How can the Pharma KingFisher Flex magnetic head be changed?

This can be done by running the Change magnet protocol under the Maintenance menu using the “up” and “down” cursor keys.
Will the Pharma KingFisher Flex magnet get weaker? If so, how long can it be used?

The Pharma KingFisher Flex magnet is made of material that is very stable. The magnetic field will not get weaker. However, extreme mechanical force or heating can cause damage to the magnet.
Caution: It is very important to keep the Pharma KingFisher Flex magnetic heads away from each other and from other magnets at all times. Clashing together of the magnets may cause serious damage to the magnets.

Can I use plates from other manufacturers with the Pharma KingFisher Flex 96 Deep-Well Magnetic Particle Processor?

No, we strongly recommend that you use Pharma KingFisher plates since they were specifically designed to be used with Pharma KingFisher Flex 96 Deep-Well Tip Combs to attain maximal performance. Plates from other manufacturers may not be compatible with the Pharma KingFisher Flex heating block. They may also cause unexpected problems, such as cross-contamination, due to the divergent well volume and bottom height of the plate.
What consumables do I need to buy for using the Pharma KingFisher Flex 96 Deep-Well Magnetic Particle Processor?

The consumables needed for the Pharma KingFisher Flex 96 Deep-Well Magnetic Particle Processor are listed below:

- Pharma KingFisher Flex 96 Deep-Well Plates (Cat. No. A43075)
- Pharma KingFisher Flex 96 Standard Plates (Cat. No. A43076)
- Pharma KingFisher Flex Magnetic Head for 96 Deep-Well Plate (Cat. No. A31542)
- Pharma KingFisher Flex 96 Deep-Well Tip Combs (Cat. No. A43074)

For Research Use Only. Not for use in diagnostic procedures.