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Gibco™ Penicillin Streptomycin GlutaMAX™ Supplement (100X)

Catalog No. A5873601
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A5873601 100 mL
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Catalog No. A5873601 Supplier Gibco™ Supplier No. A5873601
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Description

Gibco Penicillin Streptomycin GlutaMAX Supplement is a form of GlutaMAX Supplement that contains the antibiotics penicillin (10,000 units) and streptomycin (10,000 μg), along with 200 mM L-alanyl-L-glutamine dipeptide in a citrate buffer to maintain penicillin potency.

Gibco Penicillin Streptomycin GlutaMAX Supplement is a form of GlutaMAX Supplement that contains the antibiotics penicillin (10,000 units) and streptomycin (10,000 μg), along with 200 mM L-alanyl-L-glutamine dipeptide in a citrate buffer to maintain penicillin potency. GlutaMAX Supplement is an alternative to L-glutamine, with increased stability that improves cell health.

Compared to L-glutamine, GlutaMAX Supplement:

  • Minimizes toxic ammonia build-up
  • Improves cell viability and growth

Minimizes toxic ammonia build-up

Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth

Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.

About penicillin and streptomycin

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin acts by binding to the 30S subunit of the bacterial ribosome, leading to the inhibition of protein synthesis and death in susceptible bacteria.

Specifications

Concentration 100X
Form Clear, colorless to slight yellow solution
Product Type Antibiotic
Sterility Sterile-filtered
Sterilization Method 0.2 μm Sterile-filtered
With Additives GlutaMAX
Without Additives None
Formulation Liquid, citrate buffer
Osmolality 330 to 370 mOsm/kg
pH 4.7 to 6.2
Product Line GlutaMAX
Quantity 100 mL
Regulatory Status RUO
Shipping Condition -5°C to -20°C
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Frequently Asked Questions (FAQs)

What is the concentration of each component in Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) contains the antibiotics penicillin (10,000 units) and streptomycin (10,000 µg), along with 200 mM L-alanyl-L-glutamine dipeptide, GlutaMAX supplement, in a citrate buffer to maintain penicillin potency.
What is the difference between Penicillin-Streptomycin-Glutamine (100X) (Cat. No. 10378016) and Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) is an alternative to Penicillin-Streptomycin-Glutamine (100X) (Cat. No. 10378016), with increased stability that improves cell health. In aqueous solutions such as cell culture media, L-glutamine spontaneously degrades, generating toxic ammonia and pyrrolidine carboxylic acid as byproducts. GlutaMAX supplement is a dipeptide, L-alanyl-L-glutamine, which is more stable in cell culture media.
When I received Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601), the supplement was partially thawed in the bottle. Will it still work?

Yes, the product will still work. The bottle can be stored at 4 degrees C overnight to finish thawing and then aliquoted the next day for storage at -20 degrees C. Alternatively, the bottle can be stored at -20 degrees C until it is needed.
Our -20 degrees C storage space is full. Can I store Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) at -80 degrees C instead?

Yes, Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) may be stored at -80 degrees C.
How often should medium changes be performed for cells cultured in medium prepared with Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

Medium changes should be performed at least once every three days as penicillin and streptomycin are stable for three days at 37 degrees C.
How long is Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) stable in solution?

Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) is stable for two weeks in prepared cell culture media stored at 4 degrees C.
Can I use Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) in combination with other antibiotic reagents?

This would need to be empirically determined for each cell line testing each combination with other antibiotics. Penicillin and streptomycin are effective against gram-negative and gram-positive bacteria.
Will Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) affect the expansion rate of cells?

When Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) is diluted to 1X, it is designed to have equivalent performance to 1X dilutions of Penicillin-Streptomycin (100X) (Cat. No. 15140122) and GlutaMAX Supplement (100X) (Cat. No. 35050061). Cells previously maintained in medium containing 1X dilutions of Penicillin-Streptomycin (100X) and GlutaMAX Supplement (100X) should have the same rate of expansion when transitioned to Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601).
What is the shelf life of Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

The shelf life of Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) is 12 months.
How should I store Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

We recommend storing Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) at -20 degrees C until the first thaw. Upon thawing the entire contents of the bottle, the supplement can be aliquoted at appropriate volumes into small tubes for convenient future use and the aliquots can be stored at -20 degrees C. It is best to aliquot what is required for single-use experiments prior to storage at -20 degrees C.
How should I thaw Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

For convenience, we recommend thawing the entire bottle of Penicillin Streptomycin GlutaMAX Supplement (100X) at 4 degrees C overnight.
What is the proper dilution to use for Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601)?

Penicillin Streptomycin GlutaMAX Supplement (100X) (Cat. No. A5873601) is intended to be used at a 1:100 dilution as a direct replacement for Penicillin-Streptomycin (100X) (Cat. No. 15140122) and GlutaMAX Supplement (100X) (Cat. No. 35050061). Penicillin Streptomycin GlutaMAX Supplement (100X) is also an alternative for Penicillin-Streptomycin-Glutamine (100X) (Cat. No. 10378016).

For Research Use Only. Not for use in diagnostic procedures.