Recombinant Protein;Biotinylated Human PD-L1 / B7-H1 Protein, Fc Tag, Avi Tag (Avitag™);200UG;Biotinylated Human PD-L1, Fc Tag (PDL-H82F2) is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 g/mL (100 L/well) can bind Biotinylated Human PD-L1, Fc Tag (Cat. No. PDL-H82F2) with a linear range of 10-313 ng/mL (QC tested).;Biotinylated Human PD-L1Cat. No.PDL-H82F2at 1 g/mL (5 L/well) can bind Human PD-1 (Cat. No.PD1-H5221) with a linearrange of0.04-1.25 g/mL(Routinely tested).;AlphaLISAshows that the binding ofBiotinylated Human PD-L1Cat. No.PDL-H82F2toHuman PD-1 (Cat. No.PD1-H5221)was inhibited by increasing concentratio
