Thermo Scientific™ PageRuler™ Unstained High Range Protein Ladder
A mixture of 8 unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Manufacturer: Thermo Scientific™ 26637
The Thermo Scientific™ PageRuler Unstained High Range Protein Ladder is a mixture of 8 unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.The protein MW markers in this ladder resolve into clearly identifiable, sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
Size range 8 proteins spanning 60 to 250kDa
Ready-to-use supplied in a loading buffer for direct loading on gels
Sharp bands excellent accuracy for detection with coomassie or silver stains
Reference band 150kDa band has greater intensity for easy orientation
Tagged each protein in the ladder contains an integral Strep-tag™ II Sequence
Quality tested each lot evaluated by SDS-PAGE and Western blotting
Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol
Accurate protein sizing on SDS-polyacrylamide gels and Western blots
|PageRuler Unstained High Range Protein Ladder|
|8 proteins from 60 to 250kDa in reducing sample buffer|
|50 to 100 mini gel lanes at 5 to 10μL per lane|
|2 × 250mL|