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  6. Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

Catalog No. PI26630
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2 x 250 μL
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PI26630 2 x 250 μL
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Catalog No. PI26630 Supplier Thermo Scientific™ Supplier No. LSG26630
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Description

A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

The protein MW markers in this ladder resolve into clearly identifiable sharp bands ranging in size from 5kDa to 250kDa when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100, 50 and 20kDa protein bands are of greater intensity and serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5kDa peptide) contain an integral Strep-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range – eleven proteins spanning 5 to 250kDa
  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
  • Sharp bands – excellent accuracy for detection in with coomassie or silver stains
  • Reference bands – the 100, 50 and 20kDa bands are more intense for easy orientation
  • Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence
  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Specifications

Content And Storage Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Storage: Upon receipt store at -20°C
Number of Markers 11
Ready to Load Yes
Size Range 5 to 250 kDa
Gel Compatibility Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol) 250, 150, 100, 70, 50, 40, 30, 20, 15, 10, 5 kDa
Quantity 2 x 250 μL
Shipping Condition Approved for shipment on Wet or Dry Ice
Product Line PageRuler
Product Type Protein Ladder
Stain Type Unstained
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.