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  6. Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Catalog No. p-4529994
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PI26616 2 x 250 μL
PI26617 10 x 250 μL
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Catalog No. PI26616 Supplier Thermo Scientific™ Supplier No. 26616
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Description

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting.

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Applications

  • Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
  • Monitoring protein transfer onto membranes after western blotting
  • Sizing of proteins on SDS-polyacrylamide gels and western blots

Specifications

Content And Storage

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol

Storage: Upon receipt store at -20°C
Detection Method Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Number of Markers 10
Ready to Load Yes
Size Range 10 to 180 kDa
Gel Compatibility Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol) 180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
Quantity 2 x 250 μL
Shipping Condition Approved for shipment on Wet or Dry Ice
Product Line PageRuler
Product Type Protein Ladder
Stain Type Blue, Orange, Green
System Type Western Blotting, SDS-PAGE
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For Research Use Only. Not for use in diagnostic procedures.