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Gibco™ OptiPRO™ SFM AGT™ Medium

Catalog No. A4000164002
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OptiPRO™ SFM AGT™ is a serum free culture medium in a easy-to-use granular format for growth of several kidney derived cell lines MDBK, MDCK, PK-15, and VERO.

Gibco OptiPRO SFM AGT Medium is a flexible-format medium that is equivalent to OptiPRO SFM with the addition of Advanced Granulation Technology for simplified preparation. OptiPRO SFM is a serum-free, animal-origin-free culture medium designed for growth of several kidney-derived cell lines, including MDBK, MDCK, PK-15, and VERO, for virus or recombinant protein production. In addition, OptiPRO SFM has been used to successfully grow other cell lines, such as Hela, BHK-21, and COS-7.

Features of OptiPRO SFM AGT Medium include:

  • Serum-free, animal-origin-free, low-protein formulation
  • Easy adaptation for many popular cell lines
  • Scalability in flasks, cell factories, and bioreactors with AGT

Serum-free, animal-origin-free, low-protein formulation

OptiPRO SFM is formulated with a very low protein concentration (< 10 μg/mL), for easier purification of recombinant proteins.

Easy adaptation for many popular cell lines

OptiPRO SFM was designed for growth of kidney-derived cell lines such as MDCK, MDBK, VERO, and PK-15. But it has also been used successfully to grow other cell lines, such as Hela, BHK-21, and COS-7, allowing the use of a single medium for multiple cell lines. OptiPRO SFM is formulated without L-glutamine for greater stability and extended shelf life. Simply add L-glutamine or Glutamax supplement as needed.

cGMP manufacturing and quality system

OptiPRO SFM is manufactured in a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Scalability with Advanced Granulation Technology

Advanced Granulation Technology (AGT) helps simplify medium preparation at large scale with fast rehydration, a just-add-water protocol, and low dust generation, creating less pressure build-up during sterile filtration.

Specifications

Cell Line BHK-21, Vero, MDCK, MDBK, PK-15, COS-7, HeLa
Cell Type Mammalian Cells
Classification Animal Origin-free, Low Protein, Serum-free, cGMP
Concentration 1 X
Form Powder (AGT)
Product Type Media
Serum Level Serum-free
Sterility Non-sterile
Sterilization Method Non-sterile
With Additives Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine
Without Additives No Glutamine
Quantity 100 L
Formulation Proprietary
Culture Type Adherent Cell Culture
Expression System Mammalian
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.