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Gibco™ OptiPRO™ SFM
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Catalog No. 12309050
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Description

OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production.

OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7. Gibco™ OptiPRO™ SFM features:

  • Serum-free, animal origin-free, low protein formulation
  • Direct adaptation for many popular cell lines

Serum-free, animal origin-free, low protein formulation

Gibco™ OptiPRO™ SFM is formulated with a very low protein concentration (< 10 μg/mL), allowing for easier purification of your recombinant protein.

Direct adaptation for many popular cell lines

Gibco™ OptiPRO™ SFM has been designed for growth of kidney-derived cell lines such as MDCK, MDBK, VERO and PK-15. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7, allowing you to choose a single medium for multiple cell lines.

cGMP Manufacturing and Quality System

Gibco™ OptiPRO™ SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Cell Line HeLa, MDCK, BHK-21, PK-15, COS-7, MDBK, VERO
Classification Animal Origin-free, Low Protein, Serum-free
Concentration 1 X
Form Liquid
Product Type Serum Free Medium
Serum Level Serum-free
With Additives Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine
Without Additives No Glutamine
Product Line Gibco
Quantity 100 mL
Shipping Condition Room Temperature
Endotoxin Level Low
Format Bottle(s)
Included Antibiotics No antibiotics
Content And Storage Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
Culture Type Adherent Cell Culture
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Frequently Asked Questions (FAQs)

Do you offer a protocol for titering virus in OptiPRO SFM?

For virus titering in OptiPRO SFM we recommend using Falcon or equivalent 96-well plates and plating cells at a concentration of 1 X 10^6/mL. If you are having trouble growing cells for virus titration in OptiPRO SFM in the 96-well plates, we suggest switching to the EMEM with 5%FBS or DMEM with 5% FBS.
Is there a recommended protocol for culturing cell lines in OptiPRO SFM that require sequential adaptation?

The sequential adaptation process is a multi-subculture of cells. We recommend the following passaging sequence:
• First passage: use 25% OptiPRO SFM:75% original stock medium
• Second passage: use 50% OptiPRO SFM:50% original stock medium
• Third passage: use 75% OptiPRO SFM:25% original stock medium
• Fourth and subsequent passages: use 100% OptiPRO SFM
Can I add antibiotics to OptiPRO SFM?

You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)
What concentration of L-glutamine or GlutaMAX-I should I add to OptiPRO SFM before I use it?

We recommend adding L-glutamine or GlutaMAX-I to OptiPRO SFM at a final concentration of 4mM.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.