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Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311

Catalog No. LC5317
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LC5317 1 kit
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Catalog No. LC5317 Supplier Invitrogen™ Supplier No. LC5317
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Description

Includes

Each kit includes Novex IEF Anode Buffer, 50X (LC5300), Novex IEF Cathode Buffer pH 3-10, 10X (LC5310), Novex IEF Sample Buffer pH 3-10, 2X (LC5311)

Optimized for using with Novex™ pH 3-10 IEF gels

  • Concentration: 20X

Isoelectric Focusing, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Specifications

Buffer Anode & Cathode Buffers
Concentration 20 X
Gel Type IEF
pH Range 3 to 10
Gel Compatibility Novex™ IEF Gels
Quantity 1 kit
Product Line Novex
Product Type IEF Buffer Kit

Frequently Asked Questions (FAQs)

When staining IEF gels with the Colloidal Blue Staining Kit, is it necessary to use the fixing solution?

Yes, the fix serves two purposes: it fixes the sample in the IEF gel and it helps to remove gel background.

If you do not use the fixing solution, the background on the gels will be high and detection will be less sensitive.

High background is caused by ampholytes remaining in the gel.
When running an IEF gel, is it okay to use just arginine, instead of a mix of arginine and lysine, in the cathode buffer? Does it matter whether the D, L or D/L form of arginine is used?

Yes, you can use just arginine (either form of arginine) as long as the arginine free base form is used and not the hydrochloric form. Note that the lysine used to make the mixed buffer should be free base lysine; free acid lysine should not be used as a substitute. The reason for adding basic amino acids to the cathode buffer is to increase the ionic strength of the buffer in order to fortify the basic end.

For Research Use Only. Not for use in diagnostic procedures.