Invitrogen™ NuPAGE™ Large Protein Blotting Kit 

Description

The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards offer a time-saving load-and-go format. The HiMark Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460kDa in NuPAGE Tris-Acetate Gel/SDS buffer system. It's suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins. The HiMark Unstained Standard, along with the NuPAGE Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems. Visualize with Coomassie™ stain, silver, and fluorescent protein stains post-electrophoresis. Several NuPAGE Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for high-resolution large protein separation and analysis. Each kit includes two boxes of NuPAGE 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark Standard.

• Gel Percentage: 3-8 %
• Gel Size: Mini (8 x 8 cm)
• Well Format: 1D Well

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting